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Research has been conducted on Pseudomonas putida PAO1 alkane hydroxylases. The ability of the alkane hydroxylases to oxidize n-alkenes has been tested via the use of different recombination hosts and the results are reported.

Research has been conducted on rubredoxins. The sequence analysis and complementation studies indicate that these rubredoxins can be divided into AlkG1-type and AlkG2 rubredoxins and the mutagenesis results show which sequence elements determine whether rubredoxins can transfer electrons to alkane hydroxylase.

Results show that the alkane hydroxylase gene of Burkholderia cepacia RR10 exhibits catabolite repression in the presence of organic acids and sugars in complex media. Data indicate that transcription of the gene is induced by alkanes of 12 to 30 carbon chain length and by alkanols.

The mechanisms that regulate the expression of Pseudomonas oleovorans alkB were analyzed in the Escherichia coli W3110 gene expression system. The levels of AlkB cytoplasmic membrane protein alkane hydroxylase were elevated by the expression of Pseudomonas oleovorans alkB gene in Escherichia coli W3110. The expression of alkB also increased phospholipid synthesis and induced the appearance of intracytoplasmic membrane structures. Furthermore, increase phospholipid synthesis was a consequence of high-level AlkB synthesis and a requirement for microbial growth.