1. Cloning of the gene encoding quinoprotein glucose dehydrogenase from Acinetobacter calcoaceticus: evidence for the presence of a second enzyme
- Author
-
Anne-Marie Cleton-Jansen, Nora Goosen, P van de Putte, and Thibaut José Wenzel
- Subjects
Glucose Dehydrogenases ,Molecular cloning ,medicine.disease_cause ,Microbiology ,Gene product ,Plasmid ,Glucose dehydrogenase ,Pseudomonas ,Escherichia coli ,medicine ,Cloning, Molecular ,Molecular Biology ,Quinoprotein glucose dehydrogenase ,chemistry.chemical_classification ,Acinetobacter ,biology ,Genetic Complementation Test ,Chromosome Mapping ,DNA Restriction Enzymes ,biology.organism_classification ,Molecular biology ,Enzyme ,Gene Expression Regulation ,chemistry ,Biochemistry ,Genes, Bacterial ,Mutation ,Pseudomonas aeruginosa ,bacteria ,Carbohydrate Dehydrogenases ,Acinetobacter calcoaceticus ,Research Article ,Plasmids - Abstract
We cloned the gene coding for the quinoprotein glucose dehydrogenase from Acinetobacter calcoaceticus. This clone complements gdh mutations in A. calcoaceticus, Pseudomonas aeruginosa, and Escherichia coli. The gene codes for a protein with an Mr of 83,000. Evidence is presented for the presence of two different glucose dehydrogenase enzymes in A. calcoaceticus: a protein with an Mr of 83,000 and a dimer of two identical subunits with an Mr of 50,000.
- Published
- 1988
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