1. Qualitative and quantitative changes in beta-1,4-glucosidase accompanying growth of Aspergillus nidulans
- Author
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D. K. Sandhu, Somesh Sharma, and P. S. Bagga
- Subjects
chemistry.chemical_classification ,biology ,beta-Glucosidase ,fungi ,Wild type ,Parallel study ,General Medicine ,Fungi imperfecti ,Somatic hyphae ,biology.organism_classification ,Applied Microbiology and Biotechnology ,Conidium ,Spore ,Isoenzymes ,Enzyme ,Aspergillus ,chemistry ,Biochemistry ,Aspergillus nidulans - Abstract
A comparative study of the wild type A. nidulans and a mutant strain aco-T69 (lacking conidia and cleistothecia) revealed the better production of beta-1,4-glucosidase in the former. The relative distribution of the enzyme levels in various morphological structures viz. somatic hyphae, spores and cleistothecia also showed a variation. Highest specific activity was found in the cleistothecial extracts. The electrophoretic analysis of the wild type strain demonstrated the presence of three isoenzymes of beta-1,4-glucosidase i.e. beta-GLU I, beta-GLU II and beta-GLU III, the number of which varies from one to three during the growth of the organism. The parallel study of the mutant strain showed complete absence of beta-GLU II. All three forms were present in the somatic hyphae and cleistothecial extracts while spore extracts depicted the absence of beta-GLU III in the wild type.
- Published
- 1990