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Your search keyword '"Brunzelle, Joseph"' showing total 11 results
Start Over Author "Brunzelle, Joseph" Journal journal of biological chemistry
11 results on '"Brunzelle, Joseph"'

2. Phosphorylation of Cytochrome c Threonine 28 Regulates Electron Transport Chain Activity in Kidney

Author

Mahapatra, Gargi, primary, Varughese, Ashwathy, additional, Ji, Qinqin, additional, Lee, Icksoo, additional, Liu, Jenney, additional, Vaishnav, Asmita, additional, Sinkler, Christopher, additional, Kapralov, Alexandr A., additional, Moraes, Carlos T., additional, Sanderson, Thomas H., additional, Stemmler, Timothy L., additional, Grossman, Lawrence I., additional, Kagan, Valerian E., additional, Brunzelle, Joseph S., additional, Salomon, Arthur R., additional, Edwards, Brian F.P., additional, and Hüttemann, Maik, additional

Published
2017
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3. Structural and Functional Study of d-Glucuronyl C5-epimerase

Author

Qin, Yi, primary, Ke, Jiyuan, additional, Gu, Xin, additional, Fang, Jianping, additional, Wang, Wucheng, additional, Cong, Qifei, additional, Li, Jie, additional, Tan, Jinzhi, additional, Brunzelle, Joseph S., additional, Zhang, Chenghai, additional, Jiang, Yi, additional, Melcher, Karsten, additional, Li, Jin-ping, additional, Xu, H.Eric, additional, and Ding, Kan, additional

Published
2015
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8. Chaperones of F1-ATPase

Author

Ludlam, Anthony, primary, Brunzelle, Joseph, additional, Pribyl, Thomas, additional, Xu, Xingjue, additional, Gatti, Domenico L., additional, and Ackerman, Sharon H., additional

Published
2009
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9. Crystal Structures of YkuI and Its Complex with Second Messenger Cyclic Di-GMP Suggest Catalytic Mechanism of Phosphodiester Bond Cleavage by EAL Domains

Author

Minasov, George, primary, Padavattan, Sivaraman, additional, Shuvalova, Ludmilla, additional, Brunzelle, Joseph S., additional, Miller, Darcie J., additional, Baslé, Arnaud, additional, Massa, Claudia, additional, Collart, Frank R., additional, Schirmer, Tilman, additional, and Anderson, Wayne F., additional

Published
2009
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11. Structural and Functional Study of D-Glucuronyl C5-epimerase.

Author

Yi Qin, Jiyuan Ke, Xin Gu, Jianping Fang, Wucheng Wang, Qifei Cong, Jie Li, Jinzhi Tan, Brunzelle, Joseph S., Chenghai Zhang, Yi Jiang, Melcher, Karsten, Jin-ping Li, Xu, H. Eric, and Kan Ding

Subjects
*HEPARAN sulfate, *GLYCOSAMINOGLYCANS, *CELL membranes, *EXTRACELLULAR matrix, *MUTAGENESIS
Abstract

Heparan sulfate (HS) is a glycosaminoglycan present on the cell surface and in the extracellular matrix, which interacts with diverse signal molecules and is essential for many physiological processes including embryonic development, cell growth, inflammation, and blood coagulation. D-Glucuronyl C5-epimerase (Glce) is a crucial enzyme in HS synthesis, converting D-glucuronic acid to L-iduronic acid to increase HS flexibility. This modification of HS is important for protein ligand recognition. We have determined the crystal structures of Glce in apo-form (unliganded) and in complex with heparin hexasaccharide (product of Glce following O-sulfation), both in a stable dimer conformation. A Glce dimer contains two catalytic sites, each at a positively charged cleft in C-terminal α-helical domains binding one negatively charged hexasaccharide. Based on the structural and mutagenesis studies, three tyrosine residues, Tyr468, Tyr528, and Tyr546, in the active site were found to be crucial for the enzymatic activity. The complex structure also reveals the mechanism of product inhibition (i.e. 2-O- and 6-O-sulfation of HS keeps the C5 carbon of L-iduronic acid away from the active-site tyrosine residues). Our structural and functional data advance understanding of the key modification in HS biosynthesis. [ABSTRACT FROM AUTHOR]

Published
2015
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