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Your search keyword '"Hashizume, Kiyoshi"' showing total 8 results
Start Over Author "Hashizume, Kiyoshi" Journal journal of biological chemistry
8 results on '"Hashizume, Kiyoshi"'

5. Functional Interaction between Oct-1 and Retinoid X Receptor

Author

Kakizawa, Tomoko, primary, Miyamoto, Takahide, additional, Ichikawa, Kazuo, additional, Kaneko, Atsuko, additional, Suzuki, Satoru, additional, Hara, Masahiro, additional, Nagasawa, Takeshi, additional, Takeda, Teiji, additional, Mori, Jun-ichiro, additional, Kumagai, Mieko, additional, and Hashizume, Kiyoshi, additional

Published
1999
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8. Vascular endothelial cell growth factor attenuates actions of transforming growth factor-beta in human endothelial cells.

Author

Yamauchi K, Nishimura Y, Shigematsu S, Takeuchi Y, Nakamura J, Aizawa T, and Hashizume K

Subjects
Androstadienes pharmacology, Blotting, Western, Cell Line, Cells, Cultured, DNA-Binding Proteins metabolism, Dose-Response Relationship, Drug, Endothelium, Vascular cytology, Genes, Reporter, Humans, Immunoblotting, Immunoprecipitation, Luciferases metabolism, Phosphorylation, Plasmids metabolism, Plasminogen Activator Inhibitor 1 biosynthesis, Promoter Regions, Genetic, Protein Kinase Inhibitors pharmacology, Recombinant Proteins chemistry, Signal Transduction, Smad2 Protein, Smad3 Protein, Time Factors, Trans-Activators metabolism, Transcription, Genetic, Transfection, Umbilical Cord cytology, Vascular Endothelial Growth Factor A metabolism, Wortmannin, Endothelium, Vascular metabolism, Transforming Growth Factor beta metabolism, Vascular Endothelial Growth Factor A physiology
Abstract

Because vascular endothelial cell growth factor (VEGF) and transforming growth factor-beta (TGF-beta) are both involved in cellular growth and differentiation, we examined whether VEGF modifies TGF-beta signaling cascade in human umbilical cord vein endothelial cells (HUVEC). Production of plasminogen activator inhibitor-1 (PAI-1), which is under the specific control of TGF-beta, was strongly enhanced (3.5-fold) by TGF-beta treatment. Remarkably, physiological concentration of VEGF (30 nm) profoundly (by 60%) attenuated the TGF-beta stimulation of PAI-1 production without an effect on the basal PAI-1 production. In HUVECs transiently transfected with an expression construct containing a PAI-1 promoter fused to luciferase reporter gene, TGF-beta-stimulation of transcription of PAI-1 was clearly (by 60%) inhibited by VEGF. TGF-beta phosphorylation of Smad2/3, an obligatory step of intracellular TGF-beta signaling, was also suppressed by VEGF. VEGF attenuation of TGF-beta action was also demonstrated in two other endothelial cell lines. In conclusion, VEGF attenuates TGF-beta action in the human endothelial cell, specifically at the level of transcription of PAI-1 gene and Smad2/3 phosphorylation.

Published
2004
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