Search

Your search keyword '"Hattori, Shunji"' showing total 9 results
Start Over Author "Hattori, Shunji" Journal journal of biological chemistry
9 results on '"Hattori, Shunji"'

2. The dipeptide prolyl-hydroxyproline promotes cellular homeostasis and lamellipodia-driven motility via active β1-integrin in adult tendon cells

Author

Ide, Kentaro, primary, Takahashi, Sanai, additional, Sakai, Keiko, additional, Taga, Yuki, additional, Ueno, Tomonori, additional, Dickens, David, additional, Jenkins, Rosalind, additional, Falciani, Francesco, additional, Sasaki, Takako, additional, Ooi, Kazuhiro, additional, Kawashiri, Shuichi, additional, Mizuno, Kazunori, additional, Hattori, Shunji, additional, and Sakai, Takao, additional

Published
2021
Full Text
View/download PDF

3. Cyclophilin-B Modulates Collagen Cross-linking by Differentially Affecting Lysine Hydroxylation in the Helical and Telopeptidyl Domains of Tendon Type I Collagen

Author

Terajima, Masahiko, primary, Taga, Yuki, additional, Chen, Yulong, additional, Cabral, Wayne A., additional, Hou-Fu, Guo, additional, Srisawasdi, Sirivimol, additional, Nagasawa, Masako, additional, Sumida, Noriko, additional, Hattori, Shunji, additional, Kurie, Jonathan M., additional, Marini, Joan C., additional, and Yamauchi, Mitsuo, additional

Published
2016
Full Text
View/download PDF

5. Activin A Binds to Perlecan through Its Pro-region That Has Heparin/Heparan Sulfate Binding Activity

Author

Li, Shaoliang, primary, Shimono, Chisei, additional, Norioka, Naoko, additional, Nakano, Itsuko, additional, Okubo, Tetsuo, additional, Yagi, Yoshiko, additional, Hayashi, Maria, additional, Sato, Yuya, additional, Fujisaki, Hitomi, additional, Hattori, Shunji, additional, Sugiura, Nobuo, additional, Kimata, Koji, additional, and Sekiguchi, Kiyotoshi, additional

Published
2010
Full Text
View/download PDF

7. Tracing Conformational Transition of Abnormal Prion Proteins during Interspecies Transmission by Using Novel Antibodies

Author

Ushiki-Kaku, Yuko, primary, Endo, Ryo, additional, Iwamaru, Yoshifumi, additional, Shimizu, Yoshihisa, additional, Imamura, Morikazu, additional, Masujin, Kentaro, additional, Yamamoto, Takuji, additional, Hattori, Shunji, additional, Itohara, Shigeyoshi, additional, Irie, Shinkichi, additional, and Yokoyama, Takashi, additional

Published
2010
Full Text
View/download PDF

9. Activin A Binds to Perlecan through Its Pro-region That Has Heparin/Heparan Sulfate Binding Activity.

Author

Shaoliang Li, Shimono, Chisei, Norioka, Naoko, Nakano, Itsuko, Okubo, Tetsuo, Yagi, Yoshiko, Hayashi, Maria, Sato, Yuya, Fujisaki, Hitomi, Hattori, Shunji, Sugiura, Nobuo, Kimata, Koji, and Sekiguchi, Kiyotoshi

Subjects
*HEPARIN, *TRANSFORMING growth factors-beta, *EXTRACELLULAR matrix, *EMBRYOLOGY, *HOMEOSTASIS, *PROTEOGLYCANS
Abstract

Activin A, a member of the transforming growth factor-β family, plays important roles in hormonal homeostasis and embryogenesis. In this study, we produced recombinant human activin A and examined its abilities to bind to extracellular matrix proteins. Recombinant activin A expressed in 293-F cells was purified as complexes of mature dimeric activin A with its pro-region. Among a panel of extracellular matrix proteins tested, recombinant activin A bound to perlecan and agrin, but not to laminins, nidogens, collagens I and IV, fibronectin, and nephronectin. The binding of recombinant activin A to perlecan was inhibited by heparin and high concentrations of NaCl and abolished by heparitinase treatment of perlecan, suggesting that activin A binds to the heparan sulfate chains of perlecan. In support of this possibility, recombinant activin A was capable of directly binding to heparin and heparan sulfate chains. Site-directed mutagenesis of recombinant activin A revealed that clusters of basic amino acid residues, Lys259-Lys263 and Lys270-Lys272, in the pro-region were required for binding to perlecan. Interestingly, deletion of the peptide segment Lys259-Gly277 containing both basic amino acid clusters from the pro-region did not impair the activity of activin A to stimulate Smad-dependent gene expressions, although it completely ablated the perlecan-binding activity. The binding of activin A to basement membrane heparan sulfate proteoglycans through the basic residues in the pro-region was further confirmed by in situ activin A overlay assays using frozen tissue sections. Taken together, the present results indicate that activin A binds to heparan sulfate proteoglycans through its pro-region and thereby regulates its localization within tissues. [ABSTRACT FROM AUTHOR]

Published
2010
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results