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Your search keyword '"Johansen, Finn-Eirik"' showing total 6 results
Start Over Author "Johansen, Finn-Eirik" Journal journal of biological chemistry
6 results on '"Johansen, Finn-Eirik"'

6. Molecular Requirements for Sorting of the Chemokine lnterleukin-8/CXCL8 to Endothelial Weibel-Palade Bodies.

Author

HoI, Johanna, KüchIer, Axel M., Johansen, Finn-Eirik, DaIhus, Bjørn, HaraIdsen, Guttorm, and øynebråten, Inger

Subjects
*INFLAMMATORY mediators, *BIOLOGICAL transport, *VON Willebrand factor, *BLOOD platelets, *CHEMOKINES
Abstract

Sorting of proteins to Weibel-Palade bodies (WPB) of endothelial cells allows rapid regulated secretion of leukocyte-recruiting P-selectin and chemokines as well as procoagulant von Willebrand factor (VWF). Here we show by domain swap studies that the exposed aspartic acid in loop 2 (Ser44-Asp45-Gly46) of the CXC chemokine interleukin (IL)-8 is crucial for targeting to WPB. Loop 2 also governs sorting of chemokines to a-granules of platelets, but the fingerprint of the loop 2 of these chemokines differs from that of IL-8. On the other hand, loop 2 of IL-8 closely resembles a surface-exposed sequence of the VWF propeptide, the region of VWF that directs sorting of the protein to WPB. We conclude that loop 2 of IL-8 constitutes a critical signal for sorting to WPB and propose a general role for this loop in the sorting of chemokines to compartments of regulated secretion. [ABSTRACT FROM AUTHOR]

Published
2009
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