1. A Novel Arabidopsis Gene Causes Bax-like Lethality in Saccharomyces cerevisiae.
- Author
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Kawai-Yamada, Maki, Saito, Yusuke, Lihua Jin, Ogawa, Taro, Kyung-Min Kim, Li-Hua Yu, Tone, Yoshiko, Hirata, Aiko, Umeda, Masaaki, and Uchimuya, Hirofumi
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SACCHAROMYCES cerevisiae , *ARABIDOPSIS , *APOPTOSIS , *CELL death , *ENZYMES , *GENETICS , *GENES - Abstract
Overexpression of the mammalian proapoptotic protein Bax induces cell death in plant and yeast cells. The Bax inihibitor-1 (BI-1) gene rescues yeast and plant from Bax-mediated lethality, Using the Arabidopsis BI-1 (AtBI-1) gene controlled by the GAL1 promoter as a cell death suppressor in yeast, Cdf1 (cell growth defect factor-1) was isolated from Arabidopsis cDNA library. Overexpression of Cdf1 caused cell death in yeast, whereas such an effect was suppressed by co-expression of AtBI-1. The Cdf1 protein fused with a green fluorescent protein was localized in the mitochondria and resulted in the loss of mitochondrial membrane potential in yeast. The Bax-resistant mutant BRM1 demonstrated tolerance against Cdf1-mediated lethality, whereas the Δatp4 strain was sensitive to Cdf1. Our results suggest that Cdf1 and Bax cause mitochondria-mediated yeast lethality through partially overlapped pathways. [ABSTRACT FROM AUTHOR]
- Published
- 2005
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