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Start Over Author "Voeltzel T" Journal journal of biological chemistry
2 results on '"Voeltzel T"'

1. The leukemia-associated protein Btg1 and the p53-regulated protein Btg2 interact with the homeoprotein Hoxb9 and enhance its transcriptional activation.

Author

Prévôt, D, Voeltzel, T, Birot, A M, Morel, A P, Rostan, M C, Magaud, J P, and Corbo, L

Abstract

BTG1 and BTG2 belong to a family of functionally related genes involved in the control of the cell cycle. As part of an ongoing attempt to understand their biological functions, we used a yeast two-hybrid screening to look for possible functional partners of Btg1 and Btg2. Here we report the physical and functional association between these proteins and the homeodomain protein Hoxb9. We further show that Btg1 and Btg2 enhance Hoxb9-mediated transcription in transfected cells, and we report the formation of a Hoxb9.Btg2 complex on a Hoxb9-responsive target, and the fact that this interaction facilitates the binding of Hoxb9 to DNA. The transcriptional activity of the Hoxb9.Btg complex is essentially dependent on the activation domain of Hoxb9, located in the N-terminal portion of the protein. Our data indicate that Btg1 and Btg2 act as transcriptional cofactors of the Hoxb9 protein, and suggest that this interaction may mediate their antiproliferative function.

Published
2000

2. Relationships of the antiproliferative proteins BTG1 and BTG2 with CAF1, the human homolog of a component of the yeast CCR4 transcriptional complex: involvement in estrogen receptor alpha signaling pathway.

Author

Prévôt D, Morel AP, Voeltzel T, Rostan MC, Rimokh R, Magaud JP, and Corbo L

Subjects
Amino Acid Motifs, Amino Acid Sequence, Animals, Bacterial Proteins biosynthesis, Blotting, Northern, Blotting, Western, Cell Division, Chloramphenicol O-Acetyltransferase metabolism, Cloning, Molecular, DNA, Complementary metabolism, Enzyme-Linked Immunosorbent Assay, Estrogen Receptor alpha, Gene Expression Regulation, Glutathione Transferase metabolism, HeLa Cells, Humans, Immediate-Early Proteins biosynthesis, Luciferases metabolism, Molecular Sequence Data, Neoplasm Proteins biosynthesis, Plasmids metabolism, Protein Binding, Protein Structure, Tertiary, Signal Transduction, Tissue Distribution, Transcription, Genetic, Transfection, Tumor Suppressor Proteins, Bacterial Proteins metabolism, Fungal Proteins metabolism, Genes, Tumor Suppressor, Immediate-Early Proteins metabolism, Neoplasm Proteins metabolism, Receptors, Estrogen metabolism, Ribonucleases, Saccharomyces cerevisiae Proteins, Transcription Factors metabolism
Abstract

We have reported previously the physical interaction of B-cell translocation gene proteins (BTG)1 and BTG2 with the mouse protein CAF1 (CCR4-associated factor 1) and suggested that these proteins may participate, through their association with CAF1, in transcription regulation. Here we describe the in vitro and in vivo association of these proteins with hPOP2, the human paralog of hCAF1. The physical and functional relationships between the BTG proteins and their partners hCAF1 and hPOP2 were investigated to find out how these interactions affect cellular processes, and in particular transcription regulation. We defined their interaction regions and examined their expression in various human tissues. We also show functional data indicating their involvement in estrogen receptor alpha (ERalpha)-mediated transcription regulation. We found that BTG1 and BTG2, probably through their interaction with CAF1 via a CCR4-like complex, can play both positive or negative roles in regulating the ERalpha function. In addition, our results indicate that two LXXLL motifs, referred to as nuclear receptor boxes, present in both BTG1 and BTG2, are involved in the regulation of ERalpha-mediated activation.

Published
2001
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