19 results on '"Spinas, E"'
Search Results
2. Effect of self-adjusting file and WaveOne reciprocating file on the filling ability of oval-shaped canals with thermoplasticized gutta-percha
- Author
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Generali, L., Cavani, F., Elena Righi, Murri Dello Diago, A., Spinas, E., and Giannetti, L.
- Subjects
Root Canal Filling Materials ,Dental Instruments ,Root Canal Obturation ,Humans ,Gutta-Percha ,Tooth Root ,Root Canal Preparation - Abstract
The aim of the study was to compare the effect of Self-Adjusting Files (SAF) and WaveOne Primary file with syringe and needle irrigation on the filling ability of oval-shaped root canals obturated with thermoplasticized gutta-percha. Twenty-four single root teeth with single oval-shaped root canals were distributed into two experimental homogeneous groups. One group was instrumented and cleansed using the SAF system while in the other group the WaveOne system with syringe and needle irrigation was used. After instrumentation, the roots were filled by Thermafil Obturators and TopSeal sealer. Specimens were transversally sectioned at 2-, 5- and 7-mm levels from the apex and observed under light microscope. The percentage of gutta-percha filled area (PGFA), the percentage of sealer filled area (PSFA) and the percentage of voids area (PVA) were measured for each section, moreover the percentage of completely filled sections was evaluated. At all levels, no significant differences in terms of PGFA, PSFA, PVA and percentage of completely filled canals between groups were obtained (P0.05). On the contrary, when the data were pooled, the mean PGFA in the SAF group was 95.8%, whereas it was 93.2% in the WaveOne group (P less than 0.05). The percentage of sections completely filled was 77.8% in the SAF group, and 52.8% in the WaveOne group (P less than 0.05). Overall, the use of the SAF system in oval canals allows to obtain a significantly greater complete filling than the use of the WaveOne system.
- Published
- 2018
3. SDR® vs traditional composite in class II restorations in primary molars
- Author
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Giannetti, L., Murri Dello Diago, A., and Spinas, E.
- Subjects
Male ,Caries ,Deciduous teeth ,Dental materials ,Restorative treatment ,SDR ,Endocrinology, Diabetes and Metabolism ,Immunology and Allergy ,Physiology ,Immunology ,Oncology ,Endocrinology ,Physiology (medical) ,Cancer Research ,Humans ,Female ,Dental Restoration Repair ,Child ,Composite Resins ,Molar - Abstract
This study aims to compare the clinical characteristics of a new flowable resin-based composite with those of a traditional composite when applied to deciduous molars for class II restorations. Twentyeight children between 6-12 years of age, with paired minimal Class II cavities present on their primary molars, were chosen. The paired cavities were restored with either a microhybrid composite or a new flowable resin-based composite. The composites were assigned to cavities in either the right or left side of the mouth, using random allocation tables. Each pair of restorations were clinically assessed at sixmonth intervals following the United States Public Health Service criteria. The prevalence of carious lesions was higher in maxillar molars (53.6%) compared to the mandibular molars (46.4%). At 24 months, Charlie or Delta scores in the paired groups were not seen in any patient, thus demonstrating a clinical success. The statistical analysis using Pearsons chi-squared test did not reveal significant p-values for any parameter except the proximal contact showing significant p-values for this parameter. SDR appears to be the material of choice for the restoration of deciduous teeth due to its clinical features shown at 24 months as well as its ease and speed of application.
- Published
- 2018
4. Deep infiltration for the treatment of hypomineralized enamel lesions in a patient with molar incisor hypomineralization: a clinical case
- Author
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Giannetti, L., Murri Dello Diago, A., Corciolani, E., and Spinas, E.
- Subjects
Male ,Cancer Research ,Physiology ,Immunology ,Biomimetic approach ,Molar incisor hypomineralization ,Deep infiltration ,Diabetes and Metabolism ,Paediatric dentistry ,Endocrinology ,Oncology ,Physiology (medical) ,Restorative treatment ,Endocrinology, Diabetes and Metabolism ,Immunology and Allergy ,Humans ,Dental Enamel Hypoplasia ,Dental Restoration Repair ,Dental Enamel - Abstract
This case report presents the technique of deep infiltration for the treatment of hypomineralized enamel lesions in a patient affected by molar incisor hypomineralization (MIH) with the purpose of obtaining an aesthetic improvement in the affected vestibular enamel. Deep infiltration treatment involves a sequence of steps starting with an initial mechanical abrasion of the outside surface of the enamel, followed by a phase of chemical erosion using 15% hydrochloric acid, penetration through the porosity of the enamel by a very fluid resin, and finally the repair of the thin layer of lost enamel using a composite. The aesthetic improvement of the elements affected by hypomineralized lesions is discernible immediately following treatment and becomes more accentuated with time. At the expense of minimal damage to surface enamel, deep infiltration enables treatment of all types of white spot enamel lesions, regardless of aetiology, offering the possibility of restoring an aesthetically pleasing smile for patients.
- Published
- 2018
5. Fibromyalgia and bipolar disorder: extent of comorbidity and therapeutic implications
- Author
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Di Tommaso Morrison, M C, Carinci, F, Lessiani, G, Spinas, E, Kritas, S K, Ronconi, G, Caraffa, Al, and Conti, P
- Subjects
Barrett's adenocarcinoma ,Psychotropic Drugs ,Bipolar Disorder ,Fibromyalgia ,Depression ,Esophageal cancer ,Brain ,Comorbidity ,Anxiety ,Helicobacter Pylori ,Antidepressive Agents ,NO ,Barrett's esophagus ,Esophageal cancer, Barrett's esophagus, Helicobacter Pylori, Barrett's adenocarcinoma ,Memantine ,Acetamides ,Humans ,Muscle, Skeletal ,Excitatory Amino Acid Antagonists - Abstract
Fibromyalgia (FM) is a syndrome that affects muscles and soft tissues. Presenting symptoms include chronic muscle pain, fatigue, sleep problems and psychological symptoms, including depression and anxiety. There exists strong evidence of a comorbidity between FM and Bipolar Disorder (BD). In this study, papers from 2006 to February 2016 that examined the comorbidity and etiological similarities of FM and BD were reviewed, as well as the therapeutic implications of these findings. The reviewed articles showed that an adequate psychiatric screening for BD is recommended in FM patients with depressive symptoms, in order to decrease administration of antidepressants for BD, due to the lack of proven efficacy, and to limit antidepressant-induced mania. Alternative therapies, such as agomelatine, memantine and psychotherapic treatment should be considered.
- Published
- 2017
6. Delayed tooth replantation and inflammatory root resorption in childhood and adolescence.
- Author
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Spinas E, Generali L, Mameli A, Demontis C, Martinelli D, and Giannetti L
- Subjects
- Adolescent, Child, Humans, Root Resorption, Tooth Replantation
- Abstract
Avulsion of one or more permanent teeth represents an emergency in dentistry. The main treatment is the replantation of the tooth/teeth as soon as possible to decrease possible complications. However, this is not always possible, and, in many cases, the patient undergoes a delayed replantation and subsequently prosthetic and implant treatments.
- Published
- 2019
7. Tooth avulsion with extra oral time in less than 60 minutes: two different therapeutic protocols with 13-year follow-up.
- Author
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Giannetti L, Spinas E, and Murri Dello Diago A
- Subjects
- Follow-Up Studies, Humans, Periodontal Ligament, Time Factors, Tooth Avulsion, Tooth Replantation
- Abstract
The aim of this study is to assess what needs to be the priority in tooth avulsion: replantation as quickly as possible and deferred endodontic treatment, or replantation and elimination of every irritating stimulus for the periodontal ligament.
- Published
- 2019
8. Implant conometric connection through innovative prosthetic abutment: biological, clinical, surgical, and prosthetic aspects.
- Author
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Giannetti L, Spinas E, Murri Dello Diago A, Consolo U, and Generali L
- Subjects
- Humans, Dental Abutments, Dental Implants
- Published
- 2019
9. "Superficial infiltration to treat white hypomineralized defects of enamel: clinical trial with 12-month follow-up.
- Author
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Giannetti L, Murri Dello Diago A, Silingardi G, and Spinas E
- Subjects
- Dental Enamel Hypoplasia pathology, Follow-Up Studies, Humans, Dental Enamel chemistry, Dental Enamel pathology, Dental Enamel Hypoplasia therapy
- Abstract
Hypomineralization represents one of the most common defects in tooth crowns. Thanks to a wide understanding of aesthetics, patients request a treatment to resolve these defects. Different techniques are available, such as crowns/veneers, traditional restorative treatments, microabrasion, whitening, remineralizing agents and infiltration technique. The objective of this trial is to assess the effectiveness of superficial infiltration with Icon (DMG, Hamburg, Germany) on the attenuation of crown hypomineralized lesions of various etiological origins with a 12-month follow-up. Seventeen patients with white defects of enamel in the aesthetic sector were selected. The infiltration procedure was carried out following the manufacturer's instructions. Intraoral photographs were taken before and directly after treatment in order to document the immediate change in colour. Check-ups were performed 1 and 12 months later. All the defects which were treated showed a degree of attenuation. The teeth affected by molar incisor hypomineralization (MIH) showed partial attenuation in 8 cases, and only in one case the defect disappeared. Regarding the post-trauma cases, 6 were partially attenuated and 2 disappeared. The post orthodontic defects disappeared in 6 cases and were attenuated in 5. All incipient caries defects were completely hidden. Four out of 6 cases of fluorosis disappeared. Diagnosis plays a key role in guiding the dental clinical selection of treatment. While it has always been possible to achieve a high level of attenuation in cases of fluorosis and lesions of caries origin, cases of MIH should probably be treated using more invasive techniques. Post-trauma lesions should be infiltrated with caution, and only after having informed the patient of the possible ineffective outcome.
- Published
- 2018
10. Oral Lichen planus.
- Author
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Giannetti L, Dello Diago AM, and Spinas E
- Subjects
- Humans, Lichen Planus, Oral
- Abstract
Oral Lichen planus (OLP) is the most frequent mucosal localization of Lichen planus, affecting about 1-2% of the population. It is associated with skin lesions in 60-70% of cases, while occurring as the only manifestations in 15-25% of patients. Six clinical forms of OLP are identified: reticular (the most common), plaque, papular, atrophic, vesicles/bullous and erosive. The evolution is chronic, especially in the erosive form. A 1% incidence of squamous-cell carcinoma has been reported, thus considering OLP as a potential premalignant condition. The etiopathogenesis is still not completely understood: genetic (HLA-DR2), immunologic (T cell-mediated) and infectious (association with viral hepatitis C, differences in oral microbiota in OLP, and bacteria internalization into infiltrating T cells and oral epithelial cells) are considered the main predisposing or provoking factors. Management is based on the severity of the lesions; topical steroids are the first-line therapy and oral glucocorticoids are used for severe erosive lesions.
- Published
- 2018
11. Mast cell and cancer with special emphasis on il-37 an anti-inflammatory and inhibitor of innate immunity: new frontiers.
- Author
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Carinci F, Lessiani G, Spinas E, Kritas SK, Ronconi G, Caraffa Al, and Conti P
- Subjects
- Animals, Humans, Immunity, Innate immunology, Inflammation immunology, Interleukin-1 immunology, Mast Cells immunology, Neoplasms immunology
- Abstract
Mast cells (MCs) are mediators of allergy and inflammation and participate in the growth of cancer cells. MCs can promote both neoangiogenesis and tumor growth. They increase in the stroma of certain tumors where they can be recruited by tumor-derived chemoattractants, such as monocyte chemotactic protein-1 (MCP-1), RANTES and stem cell factor (SCF) to selectively secrete inflammatory molecules including chemical mediators and cytokines (TNF, IL-6 and IL-1). However, MC differentiation pathways and heterogeneity in cancer are still poorly understood. Human interleukin 1 (IL-1) plays a pivotal role in the pathogenesis of many diseases and functions, including host response to microbial invasion, injury inflammatory processes, immunologic challenges and cancer. Inflammation around the tumor includes the infiltration of mast cells and facilitates cancer growth. MCs are activated by IL-1 which can be produced by certain cancer cells and stimulate the stromal cells to selectively release IL-6, contributing to the development of Th-17 cells and increasing inflammation. IL-37, mainly generated by macrophage cell line, is an IL-1 family member which binds IL-18 receptor α (IL-18Rα) chain, and acts as a natural inhibitor of immune responses. IL-37 down-regulates cJun induced by IL-1, pro-inflammatory signals and reduces the expression of the mitogen-activated protein kinase (MAPK) p38α, STAT transcription factors and p53, affecting cellular differentiation and proliferation. In the present study we report the relationship between inflammatory mast cells, cancer and the beneficial effect of IL-37.
- Published
- 2016
12. ENOX2 (or tNOX): a new and old molecule with cancer activity involved in tumor prevention and therapy.
- Author
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Ronconi G, Lessiani G, Spinas E, Kritas SK, Caraffa Al, Saggini A, Antinolfi P, Pizzicannella J, Toniato E, and Conti P
- Subjects
- Anticarcinogenic Agents pharmacology, Anticarcinogenic Agents therapeutic use, Antineoplastic Agents, Phytogenic pharmacology, Antineoplastic Agents, Phytogenic therapeutic use, Antioxidants pharmacology, Apoptosis drug effects, Biomarkers, Tumor, Capsaicin pharmacology, Capsaicin therapeutic use, Catechin analogs & derivatives, Catechin pharmacology, Catechin therapeutic use, Down-Regulation drug effects, Early Detection of Cancer, Enzyme Induction drug effects, Gene Expression Regulation, Neoplastic drug effects, Humans, Isoflavones pharmacology, Isoflavones therapeutic use, NAD physiology, NADH, NADPH Oxidoreductases antagonists & inhibitors, NADH, NADPH Oxidoreductases blood, Neoplasm Proteins antagonists & inhibitors, Neoplasm Proteins blood, Neoplasm Proteins physiology, Neoplasms enzymology, NADH, NADPH Oxidoreductases physiology, Neoplasms drug therapy, Neoplasms prevention & control
- Abstract
Cancer includes a number of related diseases due to abnormal cell proliferation that spreads to nearby tissues. Many compounds (physical, chemical and biological) have been used to try to halt this abnormal proliferation, but the therapeutic results are poor, due also to the side effects. It has been reported that ecto-nicotinamide adenine dinucleotide oxidase di-sulfide-thiol exchanger 2 (ENOX2), also known as tumor-associated nicotinamide adenine dinucleotide oxidase (tNOX), was found to be located on the cancer cell surface, essential for cancer cell growth. Capsaicin and other anti-oxidants are capable of inhibiting tNOX, causing apoptosis of cells, exerting anti-tumor activity. It is interesting that some authors reported that ENOX2 is present in the serum of cancer patients several years before the clinical symptoms of the tumor. However, this result has to be confirmed. In this article we discuss ENOX2 and its inhibition as a hope of improving cancer therapy.
- Published
- 2016
13. Endocrinology of the skin: intradermal neuroimmune network, a new frontier.
- Author
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Caraffa Al, Spinas E, Kritas SK, Lessiani G, Ronconi G, Saggini A, Antinolfi P, Pizzicannella J, Toniato E, Theoharides TC, and Conti P
- Subjects
- Adrenocorticotropic Hormone physiology, Animals, Corticotropin-Releasing Hormone physiology, Cytokines physiology, Humans, Signal Transduction physiology, Stress, Psychological physiopathology, Substance P physiology, Endocrine System physiology, Skin immunology
- Abstract
Endocrinology systems exert an important effect on vascular function and have direct actions on blood vessels. Estrogens provoke an increase in skin elasticity, epidermal hydration, skin thickness, reduce skin wrinkles and augment the content of collagen and the level of vascularisation. Therefore, there is an intricate cross-talk between skin conditions and stress. In stress, β2--adrenoreceptor (β2AR) pathway, cortisol, epinephrine and norepinephrine increase DNA damage and interfere with the regulation of the cell cycle, contributing to aging and skin diseases. Substance P is a neuropeptide released in the skin from the peripheral nerve and is related to stress and inflammation. SP provokes infiltration of inflammatory cells in the skin and induces a variety of cytokines/chemokines. Corticotropin-releasing hormone (CRH), produced by mast cells, is a neuropeptide also expressed in skin and responds to stress. CRH initiates diverse intracellular signaling pathways, including cAMP, protein kinase C, and mitogen-activated protein kinases (MAPK). Under stress, CRH, glucocorticoids, epinephrine and cytokines are generated. Moreover, the release of ACTH binds the receptor MC2-R and stimulates the generation of glucocorticoids such as corticosterone and cortisol, which interact with the transcription factors AP-1 and NF-kB. In skin keratinocytes, ACTH promotes the generation of pro-inflammatory cytokines, which enhances T-cell activity. Cortisol is immunosuppressive by inhibiting Th1 and Th2 cell response, antigen presentation, antibody and cytokine/chemokine production. However, glucocorticoids are certainly helpful in Th1-mediated autoimmune disorders. On the other hand, cytokines, such as TNF, IL-1 and IL-6, stimulate the generation of CRH and activate HPA axis in inflammatory states. Here, we describe for the first time a cross-talk between endocrinology and skin, including pro-inflammatory cytokines and neurogenic inflammatory pathways.
- Published
- 2016
14. Is vitamin E an anti-allergic compound?
- Author
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Caraffa AL, Varvara G, Spinas E, Kritas SK, Lessiani G, Ronconi G, Saggini A, Antinolfi P, Frydas I, De Tommaso Morrison MC, and Conti P
- Subjects
- Animals, Anti-Allergic Agents chemistry, Humans, Hypersensitivity drug therapy, Vitamin E chemistry, Anti-Allergic Agents therapeutic use, Vitamin E therapeutic use
- Abstract
Vitamin E is found in eight forms in nature which include four tocopherols (alpha, beta, gamma and delta) and four tocotrianols (alpha, beta, gamma and delta). The classic effect of vitamin E is to reduce and prevent oxygen damage to the tissue and is useful for the treatment of pain, inflammation and allergic reactions. In addition to antioxidant activity, vitamin E also has a number of different and related functions. It protects against cancer, improves immune response, lowers the incidence of infectious diseases, cardiovascular diseases and is protective in allergy and asthma risk, and other disorders. Vitamin E increases n-6 polyunsaturated fatty acid (PUFA) and decreases n-3 PUFA, an effect that diminishes asthma and allergic diseases. Moreover, vitamin E regulates vascular cell adhesion molecule-1 (VCAM-1)-dependent leukocyte migration through its oxidant and non-antioxidant effect. Furthermore, vitamin E modulates the endothelial function by altering VCAM-1-induced oxidative activation of endothelial cell PKCα. However, vitamin E is not consistently associated with asthma and/or allergy, and in some cases there are conflicting results on allergy and inflammatory diseases. The association of vitamin E and allergy appears to be very complex, and further study needs to clarify this dilemma.
- Published
- 2016
15. Role of TNF in mast cell neuroinflammation and pain.
- Author
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Gu Y, Yang DK, Spinas E, Kritas SK, Saggini A, Caraffa A, Antinolfi P, Saggini R, and Conti P
- Subjects
- Humans, NF-kappa B physiology, Brain Diseases etiology, Inflammation etiology, Mast Cells physiology, Pain etiology, Tumor Necrosis Factor-alpha physiology
- Abstract
Inflammatory mediators, such as cytokines, chemokines and arachidonic acid compounds, lead to vascular permeability and dilation and increase sensitization and pain receptors. Proinflammatory cytokines, including tumor necrosis factor, are involved in the etiology of clinical neurological disorders. These cytokines activate nuclear factor-κB (NF-κB) which leads to the activation of different inflammatory genes. TNF implicated in neurological disorders has an important role in the activation of microglia and astrocytes. The inhibition of TNF may lead to the decrease of microglia activation and can be useful for therapeutic intervention. TNF, at the site of nerve injury may activate mast cells (MCs) which mediate pathologic events such as headache and pain. TNF is the only cytokine stored in mast cells and can be rapidly released along with biogenic amines after MC stimulation. Activation of MCs leads to NF-κB and AP1 generation with release of many cytokines including TNF, IL-33 and IL-1. In this paper we discuss the role of TNF in MC activation, mediating pain and neurological disorders.
- Published
- 2015
16. IMMUNOMODULATORY EFFECTS OF VITAMIN D ON SKIN INFLAMMATION.
- Author
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Toniato E, Spinas E, Saggini A, Kritas SK, Caraffa A, Antinolfi P, Saggini R, Pandolfi F, and Conti P
- Subjects
- Cytokines immunology, Humans, Lymphocytes immunology, Lymphocytes pathology, MAP Kinase Signaling System drug effects, MAP Kinase Signaling System immunology, Macrophages immunology, Macrophages pathology, Mast Cells immunology, Mast Cells pathology, Receptors, Calcitriol immunology, Dermatitis drug therapy, Dermatitis immunology, Dermatitis pathology, Immunologic Factors therapeutic use, Skin immunology, Skin pathology, Vitamin D therapeutic use
- Abstract
Vitamin D has a major role in calcium absorption and maintenance of healthy bones. Vitamin D is also involved in cancer, cardiovascular system, allergic diseases, immune regulation and immune disor¬ders. Irradiation of food as well as animals produces vitamin D and more than 90% of previtamin D3 synthesis in the skin occurs in the epidermis. Vitamin D receptor has been found in many cells including T and B lymphocytes, macrophages, mast cells, NK cells and Tregs, and it selectively binds with high affinity to its ligand. Vitamin D binds its receptor VDR, resulting in transcription of a number of genes playing a role in inhibition of MAPK. Its effect may be also mediated by the direct activation of PKC. Vitamin D has the ability to suppress inflammatory cytokines such as TNF, IL-1, IFN-gamma and IL-2; while it increases the generation of anti-inflammatory cytokines IL-4 and IL-10. In B cells, vitamin D3 have also been shown to suppress IgE antibody class switch partly through the inhibition of NF-kB. Here we discuss the relationship between vitamin D, immunity and skin disorders.
- Published
- 2015
17. CROSSTALK BETWEEN VITAMIN B AND IMMUNITY.
- Author
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Spinas E, Saggini A, Kritas SK, Cerulli G, Caraffa A, Antinolfi P, Pantalone A, Frydas A, Tei M, Speziali A, Saggini R, Pandolfi F, and Conti P
- Subjects
- Animals, Cytokines biosynthesis, Cytokines physiology, Heart Failure etiology, Humans, Inflammation physiopathology, Models, Animal, Nervous System Diseases etiology, Nervous System Diseases immunology, Neuromuscular Diseases etiology, Neuromuscular Diseases immunology, Vitamin B Complex therapeutic use, Vitamin B Deficiency complications, Immune System physiology, Vitamin B Complex physiology, Vitamin B Deficiency immunology
- Abstract
Vitamin B1 (thiamin) is considered to be the oldest vitamin and in 1936 R.R. Williams and colleagues determined its chemical structure and were able to synthesize this vitamin. Vitamin B1 influences pro-apoptotic proteins, mitochondrial membrane potential, cytochrome C release, protein kinases, p38-MAPK, suppresses oxidative stress-induced NF-kappaB and has anti-inflammatory properties. Deficiency of vitamin B1 may cause beriberi, dysfunction of the nervous system, neuroinflammation, T cell infiltration, chemokine CCL2 activation, over expression of proinflammatory cytokines, such as IL-1, TNF, IL-6, and arachidonic acid products, and induces expression of CD40 by the microglia and CD40L by astrocytes which provoke the death of neurons. Here we report the relationship between vitamin B complex and immunity.
- Published
- 2015
18. Can vitamin a mediate immunity and inflammation?
- Author
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Spinas E, Saggini A, Kritas SK, Cerulli G, Caraffa A, Antinolfi P, Pantalone A, Frydas A, Tei M, Speziali A, Saggini R, Pandolfi F, and Conti P
- Subjects
- Animals, Carotenoids pharmacology, Humans, Immunity, Innate drug effects, Inflammation immunology, Phagocytosis drug effects, Phagocytosis physiology, T-Lymphocytes, Helper-Inducer drug effects, T-Lymphocytes, Helper-Inducer immunology, T-Lymphocytes, Regulatory drug effects, T-Lymphocytes, Regulatory immunology, Avitaminosis immunology, Immunity, Innate physiology, Inflammation etiology, Vitamin A pharmacology, Vitamin A physiology
- Abstract
Vitamins are natural components of foods and are organic compounds distinct from fat, carbohydrates and proteins. Vitamin A is the generic descriptor for compounds with the qualitative biological activity of retinol. Unlike beta-carotene, vitamin A is not an antioxidant and its benefit is related to possible boosting of immune reactions. The effect of vitamin A on immune function is wide-reaching and its deficiency appears to affect immunity in several ways. Innate and adaptive immune responses are affected in some way by lack of vitamin A. Retinoids seem to act on differentiation of lymphocytes, antibody production, phagocytosis of macrophages, NK, Treg, and T helper cell activity. In addition, in humans, signs of a vitamin A deficiency also include the dysregulation of cytokine/chemokine generation and release. However, excess of vitamin A has been demonstrated to have toxic effects in most species studied. Here we summarize some important effects of vitamin A in immunity and inflammation.
- Published
- 2015
19. Comparison of salivary antioxidant enzyme activity between ex-smokers and subjects who had never smoked.
- Author
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Giuca MR, Pasini M, D'Ercole S, Martinelli D, Tripodi D, and Spinas E
- Subjects
- Adult, Female, Humans, Male, Middle Aged, Smoking Cessation, Antioxidants metabolism, Glutathione Peroxidase metabolism, Saliva enzymology, Smoking, Superoxide Dismutase metabolism
- Abstract
Smoke contains oxidants such as oxygen-free radicals which are probably the major cause of damage to biomolecules. A decrease of salivary antioxidant enzymes was detected in habitual smokers. However, the effects of cigarette smoke on salivary antioxidant enzymes may persist after withdrawal from smoking. The objective of this study was to assess salivary superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activity in ex-smokers in comparison with that of subjects who had never smoked. The test group included 25 ex-smokers (13 males and 12 females; mean age: 48 ± 8 years) who had given up smoking for at least one year but for no more than 2 years, and a control group consisting of 25 subjects (14 males and 11 females; mean age: 50 ± 12 years) who had never smoked. Salivary samples were collected and SOD and GSH-Px activity was measured. Students t-test was used to evaluate differences between groups and significant differences were observed for p < 0.05. A significant decrease (p < 0.05) of GSH-Px (14.5 ± 2) was observed in the test group compared to the control group (30 ± 4). However, SOD was very similar in the two groups: 0.9 ± 0.3 in the test group and 0.8 ± 0.3 in the controls and no significant difference was detected (p> 0.05). Detoxification of hydrogen peroxide by the GSHPx was altered even after withdrawal from smoking, while the production of hydrogen peroxide, that is mediated by SOD, was not modified.
- Published
- 2015
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