1. Characterization of Vero cells
- Author
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Bonnie D. Brock, Rosalyn E. Wallace, Stephen K. Swanson, Francis R. Cano, Mary B. Ritchey, Steven Joseph Mento, Carolyn Louise Weeks-Levy, and Kieron J. Kowal
- Subjects
viruses ,Hybridization probe ,Mice, Nude ,Neoplasms, Experimental ,Biology ,Virology ,General Biochemistry, Genetics and Molecular Biology ,Reverse transcriptase ,Virus ,Mice ,Microscopy, Electron ,genomic DNA ,chemistry.chemical_compound ,Retroviridae ,chemistry ,Cell culture ,DNA, Viral ,Cell Adhesion ,Vero cell ,Animals ,Female ,Simian Immunodeficiency Virus ,African Green Monkey ,Vero Cells ,DNA - Abstract
A cell line used in the production of biologicals should be free of infectious agents, and 'described with respect to cytogenetic characteristics and tumorigenicity'. Vero, a continuous cell line derived from a normal African green monkey kidney, was examined for the presence of retroviruses and for tumorigenic potential. We were unable to detect the presence of retroviruses by reverse transcriptase assay, electron microscopy or hybridization of cellular genomic DNA with Mason-Pfizer monkey virus DNA probes. In addition, passage 156 Vero cells did not form progressively growing tumors in nude mice or grow with high efficiency in soft agarose.
- Published
- 1988
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