1. Second-harmonic imaging of cornea after intrastromal femtosecond laser ablation
- Author
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Matthias Walter, Leander Zickler, Frieder Loesel, Josef F. Bille, Meng Han, and Guenter Giese
- Subjects
Fluorescence-lifetime imaging microscopy ,Materials science ,Corneal Surgery, Laser ,Corneal Stroma ,Biomedical Engineering ,Second-harmonic imaging microscopy ,In Vitro Techniques ,law.invention ,Biomaterials ,Optics ,law ,Cornea ,Microscopy ,medicine ,Animals ,Microscopy, Confocal ,Laser ablation ,business.industry ,Second-harmonic generation ,Image Enhancement ,Laser ,eye diseases ,Atomic and Molecular Physics, and Optics ,Electronic, Optical and Magnetic Materials ,Microscopy, Fluorescence, Multiphoton ,medicine.anatomical_structure ,Surgery, Computer-Assisted ,Femtosecond ,Cattle ,Collagen ,sense organs ,business - Abstract
Nonlinear laser scanning microscopy is widely used for noninvasive imaging in cell biology and tissue physiology. However, multiphoton fluorescence imaging of dense, transparent connective tissue (e.g., cornea) is challenging since sophisticated labeling or slicing is necessary. High-resolution, high-contrast second harmonic generation (SHG) imaging of corneal tissue based on the intrinsic structure of collagen is discussed. The three-dimensional corneal ultrastructure in depths up to hundreds of microns can be probed noninvasively, without any staining or mechanical slicing. As an important application of second harmonic imaging in ophthalmology, the modification of corneal ultrastructure using femtosecond laser intrastromal ablation is systematically investigated to evaluate next-generation refractive surgical approaches.
- Published
- 2004
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