1. In vivo imaging of elastic fibers using sulforhodamine B
- Author
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Clément Ricard, Boudewijn van der Sanden, Jean-Claude Vial, Julien Douady, Grenoble Institut des Neurosciences (GIN), Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire de Spectrométrie Physique (LSP), Université Joseph Fourier - Grenoble 1 (UJF)-Centre National de la Recherche Scientifique (CNRS), Collaboration, and Serduc, Raphael
- Subjects
Sulforhodamine B ,MESH: Elastic Tissue ,Mice ,chemistry.chemical_compound ,0302 clinical medicine ,MESH: Collagen ,Microscopy ,MESH: Animals ,MESH: Muscle, Skeletal ,0303 health sciences ,biology ,Anatomy ,MESH: Fluorescent Dyes ,Fluorescence ,Atomic and Molecular Physics, and Optics ,3. Good health ,Electronic, Optical and Magnetic Materials ,medicine.anatomical_structure ,Female ,Collagen ,Preclinical imaging ,Materials science ,MESH: Rats ,MESH: Elastin ,Biomedical Engineering ,Mice, Nude ,MESH: Rhodamines ,Biomaterials ,03 medical and health sciences ,In vivo ,MESH: Mice, Nude ,medicine ,Animals ,Rats, Wistar ,Muscle, Skeletal ,MESH: Mice ,Fluorescent Dyes ,030304 developmental biology ,Rhodamines ,Epimysium ,MESH: Blood Vessels ,MESH: Rats, Wistar ,Elastic Tissue ,Elastin ,Rats ,Staining ,Microscopy, Fluorescence, Multiphoton ,chemistry ,MESH: Microscopy, Fluorescence, Multiphoton ,biology.protein ,Blood Vessels ,MESH: Female ,030217 neurology & neurosurgery ,Biomedical engineering - Abstract
International audience; Until now, the imaging of elastic fibers was restricted to tissue sections using the endofluorescence properties of elastin or histological dyes. Methods to study their morphology in vivo and in situ have been lacking. We present and characterize a new application of a fluorescent dye for two-photon microscopy: sulforhodamine B (SRB), which is shown to specifically stain elastic fibers in vivo. SRB staining of elastic fibers is demonstrated to be better than using elastin endofluorescence for two-photon microscopy. Our imaging method of elastic fibers is shown to be suitable for simultaneous imaging with both other fluorescent intravital dyes and second-harmonic generation (SHG). We illustrate these findings with intravital imaging of elastic and collagen fibers in muscle epimysium and endomysium and in blood vessel walls. We expect SRB staining to become a key method to study elastic fibers in vivo.
- Published
- 2007
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