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Your search keyword '"Group I ribozyme"' showing total 2 results
Start Over Descriptor "Group I ribozyme" Journal journal of bioscience and bioengineering
2 results on '"Group I ribozyme"'

1. Catalytic RNA nano-objects formed by self-assembly of group I ribozyme dimers serving as unit structures

Author

Junya Akagi, Yoshiya Ikawa, Masayuki Endo, Kumi Hidaka, Ryuji Kiyooka, Hiroshi Sugiyama, and Shigeyoshi Matsumura

Subjects
0106 biological sciences, 0301 basic medicine, Bioengineering, Group I ribozyme, 01 natural sciences, Applied Microbiology and Biotechnology, 03 medical and health sciences, 010608 biotechnology, Cleave, Nano, RNA, Catalytic, Catalytic RNA, biology, Chemistry, Atomic force microscopy, Ribozyme, RNA, Nanostructures, Crystallography, 030104 developmental biology, Biocatalysis, biology.protein, Nucleic Acid Conformation, Self-assembly, Dimerization, Biotechnology
Abstract

Ribozymes with modular structures are attractive platforms for the construction of nanoscale RNA objects with biological functions. We designed group I ribozyme dimers as unit ribozyme dimers (Urds), which self-assembled to form their polymeric states and also oligomeric states with defined numbers of Urds. Assembly of Urds yielded catalytic ability of a pair of distinct ribozyme units to cleave two distinct substrates. The morphologies of the assembled ribozyme structures were observed directly by atomic force microscopy (AFM).

Published
2020

2. Oligomerization of a modular ribozyme assembly of which is controlled by a programmable RNA-RNA interface between two structural modules

Author

Masayuki Endo, Hiroshi Sugiyama, Yuki Suzuki, Narumi Uehara, Yoshiya Ikawa, Ryusei Tsuruga, Hiroyuki Furuta, and Shigeyoshi Matsumura

Subjects
0106 biological sciences, 0301 basic medicine, Biochemical Phenomena, Interface (computing), Bioengineering, Group I ribozyme, Microscopy, Atomic Force, 01 natural sciences, Applied Microbiology and Biotechnology, 03 medical and health sciences, 010608 biotechnology, RNA, Catalytic, Catalytic RNA, biology, Chemistry, Atomic force microscopy, business.industry, Ribozyme, Tetrahymena, RNA, Modular design, biology.organism_classification, Combinatorial chemistry, Nanostructures, 030104 developmental biology, biology.protein, Nucleic Acid Conformation, business, Biotechnology
Abstract

Bimolecular ribozymes derived by physical dissection of unimolecular ribozymes consisting of two structural modules are promising platforms for the design and construction of assembled RNA nanostructures. Unit RNAs to be assembled intermolecularly into one-dimensional (1D) oligomers are designed by reconnecting the two structural modules in a manner different from the parent ribozymes. This strategy was applied to the Tetrahymena group I ribozyme. We constructed 1D ribozyme oligomers the assembly of which was observed by atomic force microscopy (AFM) and also controlled rationally to design a heterooctamer by differentiating the interface between the two modules.

Published
2019

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