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The industrial effluent contaminated with organic pollutants has been causing an increase in the toxicity of the ecosystem, causing a great environmental impact. Thus, the present work aims the green synthesis of silver nanoparticles (AgNPs) from Aloe vera, its characterization and antimicrobial activity against Pseudomonas aeruginosa (ATCC 27853) and Staphylococcus aureus (ATCC 25923). AgNPs were characterized by X-ray diffraction (XRD), Scanning Electronic Microscopy with Energy Dispersive Spectroscopy (SEM-EDS), Zeta Potential (ZP) and N2 porosimetry (BET/BJH method). Antimicrobial activity were carried out by Minimal Inhibitory Concentration (MIC) method. The XRD demonstrated characteristic peaks of AgNPs at 38.29°; 44.55° and 64.81°, and SEM-EDS micrographs showed that AgNPs produced by biomolecules of Aloe vera extract resulted in a weight concentration around 92.59% silver, 7.15% oxygen and 0.26% chlorine. Regarding zeta potential, all samples showed negative electric charge (around −35.3 mV), while N2 porosimetry resulted in a surface specific area of 6.09 m2 g−1, with a volume and diameter pore of 0.032 cm³ g−1 and 33.47, respectively. Antimicrobial activity was observed at 15.62 µg mL−1 and 31.25 µg mL−1 for P. aeruginosa and S. aureus, respectively. Thus, AgNPs can be considered a promising nanoparticle for degradation of organic pollutants in aqueous solution as well as an adjuvant for treatment of microbial infections.