1. Synthetic surfaces for human embryonic stem cell culture
- Author
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Sherry T. Hikita, Poornima Kolhar, Venkata Ramana Kotamraju, Dennis O. Clegg, and Erkki Ruoslahti
- Subjects
Cell Survival ,Cell Culture Techniques ,Bioengineering ,Peptide ,Biology ,Applied Microbiology and Biotechnology ,Extracellular matrix ,Humans ,reproductive and urinary physiology ,Cells, Cultured ,Embryonic Stem Cells ,Cell Proliferation ,chemistry.chemical_classification ,Tissue Engineering ,Cell growth ,RGD peptide ,General Medicine ,Anatomy ,equipment and supplies ,Embryonic stem cell ,Cell biology ,Extracellular Matrix ,Cyclic rgd peptide ,chemistry ,Cell culture ,embryonic structures ,biological phenomena, cell phenomena, and immunity ,Stem cell ,Oligopeptides ,Biotechnology - Abstract
Human embryonic stem cells (hESCs) have numerous potential biomedical applications owing to their unique abilities for self-renewal and pluripotency. Successful clinical application of hESCs and derivatives necessitates the culture of these cells in a fully defined environment. We have developed a novel peptide-based surface that uses a high-affinity cyclic RGD peptide for culture of hESCs under chemically defined conditions.
- Published
- 2009