1. miR-145 functions as tumor suppressor and targets two oncogenes, ANGPT2 and NEDD9, in renal cell carcinoma.
- Author
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Lu, Ruijing, Ji, Ziliang, Li, Xiaoqing, Zhai, Qingna, Zhao, Chunjuan, Jiang, Zhimao, Zhang, Shiqiang, Nie, Liping, and Yu, Zhendong
- Subjects
RENAL cell carcinoma ,MICRORNA genetics ,TUMOR suppressor genes ,GENE targeting ,ONCOGENES ,ANGIOPOIETIN-2 ,GENE expression ,CANCER cell proliferation ,GENETICS - Abstract
Purpose: Abnormal expression of miRNAs is closely related to a variety of human cancers. The purpose of this study is to identify new tumor suppressor miRNA and elucidate its physiological function and mechanism in renal cell carcinoma (RCC). Methods: The expression of miR-145 in 45 RCC and adjacent normal tissues was performed by quantitative RT-PCR. Cell proliferation, migration, invasion, apoptosis and cycle assays were carried out for functional analysis after miR-145 transfection. Two target genes of miR-145 were identified by luciferase reporter assay. The altered expression of 84 epithelial to mesenchymal transition (EMT)-related genes after miR-145 transfection was detected by RT Profiler EMT PCR array. Results: The expression of miR-145 was downregulated in RCC compared to their normal adjacent tissues. Restoring miR-145 expression in RCC cell lines dramatically suppressed cell proliferation, migration and invasion, and induced cell apoptosis and G2-phase arrest. We further validated those miR-145 targets two oncogenes, ANGPT2 and NEDD9 in RCC. In addition, miR-145 was found to regulate numerous genes involved in the EMT. Conclusions: These findings demonstrate that miR-145 functions as tumor suppressor in RCC, suggesting that miR-145 may be a potential therapeutic target for RCC. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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