1. Identification of side population cells in human hepatocellular carcinoma cell lines with stepwise metastatic potentials.
- Author
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Guo-Ming Shi, Yang Xu, Jia Fan, Jian Zhou, Xin-Rong Yang, Shuang-Jian Qiu, Yong Liao, Wei-Zhong Wu, Yuan Ji, Ai-Wu Ke, Zhen-Bin Ding, Yi-Zhou He, Bing Wu, Guo-Huan Yang, Wen-Zhen Qin, Wu Zhang, Jiang Zhu, Zhi-Hui Min, and Zhi-Quan Wu
- Subjects
LIVER cancer ,IMMUNOFLUORESCENCE ,CELL lines ,MICE ,STEM cells - Abstract
To identify the side population (SP) cells from four hepatocellular carcinoma (HCC) cell lines with stepwise metastatic potentials. SP cells were sorted from HCCLM3, MHCC97-H, MHCC97-L and Hep3B by flow cytometry, and then analyzed by differentiation study, clonogenic assay, chemoresistance study and tumorigenicity assay in vivo. The expression of ABCG
2 in SP cells was detected by immunocytochemistry, western blotting and real-time quantitative PCR, respectively. There was significant difference in SP proportion among HCCLM3, MHCC97-H, MHCC97-L and Hep3B (28.7 ± 1.6%, 14.5 ± 0.6%, 4.2 ± 0.4%, 0.9 ± 0.1%, respectively, P < 0.01). All the SP cells showed similar characteristics of self-renewal, high clonogenicity, remarkable chemo-resistance and high expression of ABCG2 . As low as 2,000 SP cells could initiate tumors in non-obese diabetic/severe combined immunodeficiency mice successfully. SP cells purified from HCC cell lines harbors cancer stem cell-like properties, and may be related to the metastatic potentials and therapeutic-resistance of HCC. [ABSTRACT FROM AUTHOR]- Published
- 2008
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