1. Expression of multidrug resistance-associated ABC transporters in B-CLL is independent of ZAP70 status
- Author
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Ulrich Keller, Christian Bogner, Thomas Licht, Madlen Oelsner, Thomas Decker, Alexander Hoellein, Christian Peschel, and Stefanie Hauswald
- Subjects
Adult ,Male ,Cancer Research ,medicine.medical_specialty ,Chronic lymphocytic leukemia ,ATP-binding cassette transporter ,Drug resistance ,Biology ,immune system diseases ,hemic and lymphatic diseases ,Internal medicine ,medicine ,Tumor Cells, Cultured ,Humans ,Aged ,Aged, 80 and over ,Hematology ,Blood Cells ,ZAP-70 Protein-Tyrosine Kinase ,Gene Expression Regulation, Leukemic ,ZAP70 ,Lymphoma, Non-Hodgkin ,General Medicine ,Middle Aged ,medicine.disease ,Leukemia, Lymphocytic, Chronic, B-Cell ,Lymphoma ,Multiple drug resistance ,Haematopoiesis ,Phenotype ,Oncology ,Drug Resistance, Neoplasm ,Immunology ,Cancer research ,ATP-Binding Cassette Transporters ,Female ,Multidrug Resistance-Associated Proteins - Abstract
To assess whether the poor prognosis of ZAP70-positive B-cell chronic lymphocytic leukemia (CLL) is associated with the overexpression of ABC transporter genes that are responsible for pleiotropic drug resistance. The transcript level of ten drug transporters was analyzed using semiquantitative and quantitative RT-PCR in control hematopoietic cells, in 41 CLL patient samples and in 5 lymphoma cell lines. ZAP70 status was determined by immunoblotting. Of all analyzed transporters, MDR1, MDR2, MRP1, MRP4, MRP5, and MRP7 were expressed at a significantly higher level in B lymphocytes when compared with other hematopoietic cells in peripheral blood. A subgroup of 41 CLL patient samples showed similar or higher expression of these genes than control B cells, and CLL cells exhibited high expression when compared with multiple lymphoma cell lines. No significant correlation between ZAP70 expression and ABC transporter expression was observed. The ZAP70 status is independent of the multidrug resistance phenotype in CLL.
- Published
- 2009