1. Descemet membrane air-bubble separation in donor corneas.
- Author
-
Venzano D, Pagani P, Randazzo N, Cabiddu F, and Traverso CE
- Subjects
- Aged, Aged, 80 and over, Air, Cell Count, Cryopreservation, Eye Banks, Humans, Middle Aged, Tissue Preservation, Descemet Membrane cytology, Descemet Stripping Endothelial Keratoplasty methods, Endothelium, Corneal cytology, Tissue Donors, Tissue and Organ Harvesting methods
- Abstract
We describe a technique to obtain Descemet-endothelium disks from donors. To detach Descemet membrane, an air bubble was introduced in the deep stroma of human donor corneas mounted on an artificial chamber. In Group A (n = 5), the bubble was left inflated. In Group B (n = 4), the bubble was deflated immediately after the membrane was detached. In Group C (n = 7), the Descemet-endothelium disk was trephined and separated from the stroma after the bubble was deflated. All tissues were stored at 4°C. Descemet detachment was achieved in 89% of the tissues. After 48 hours, the mean endothelial loss was 83% ± 10% (SD), 15% ± 11%, and 3% ± 3% in the 3 groups, respectively. With this technique, Descemet-endothelium disks were obtained without significant alterations in the endothelial layer., (Copyright © 2010 ASCRS and ESCRS. Published by Elsevier Inc. All rights reserved.)
- Published
- 2010
- Full Text
- View/download PDF