1. New GABAergic interneurons in the adult neocortex and striatum are generated from different precursors.
- Author
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Dayer AG, Cleaver KM, Abouantoun T, and Cameron HA
- Subjects
- Amino Acid Transport System X-AG analysis, Animals, Antigens analysis, Bromodeoxyuridine metabolism, Calbindin 2, Calbindins, Cell Movement physiology, Cell Proliferation, Corpus Striatum chemistry, Doublecortin Domain Proteins, Doublecortin Protein, ELAV Proteins, ELAV-Like Protein 3, Glutamate Decarboxylase analysis, Glutamate Plasma Membrane Transport Proteins, Immunohistochemistry, Interneurons chemistry, Isoenzymes analysis, Male, Microscopy, Fluorescence, Microtubule-Associated Proteins analysis, Neocortex chemistry, Nerve Tissue Proteins analysis, Neuropeptides analysis, Proteoglycans analysis, RNA-Binding Proteins analysis, Rats, Rats, Sprague-Dawley, S100 Calcium Binding Protein G analysis, Satellite Cells, Perineuronal chemistry, Satellite Cells, Perineuronal cytology, Symporters analysis, Corpus Striatum cytology, Interneurons cytology, Neocortex cytology, gamma-Aminobutyric Acid analysis
- Abstract
Ongoing neurogenesis in the adult mammalian dentate gyrus and olfactory bulb is generally accepted, but its existence in other adult brain regions is highly controversial. We labeled newly born cells in adult rats with the S-phase marker bromodeoxyuridine (BrdU) and used neuronal markers to characterize new cells at different time points after cell division. In the neocortex and striatum, we found BrdU-labeled cells that expressed each of the eight neuronal markers. Their size as well as staining for gamma-aminobutyric acid (GABA), glutamic acid decarboxylase 67, calretinin and/or calbindin, suggest that new neurons in both regions are GABAergic interneurons. BrdU and doublecortin-immunoreactive (BrdU+/DCX+) cells were seen within the striatum, suggesting migration of immature neurons from the subventricular zone. Surprisingly, no DCX+ cells were found within the neocortex. NG2 immunoreactivity in some new neocortical neurons suggested that they may instead be generated from the NG2+ precursors that reside within the cortex itself.
- Published
- 2005
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