1. Extracellular vesicles of stromal origin target and support hematopoietic stem and progenitor cells
- Author
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Gregoire Stik, Thierry Jaffredo, Simon Crequit, Pierre Charbord, Laurence Petit, Charles Durand, Jennifer Durant, Laboratoire de synthèse organique (DCSO), École polytechnique (X)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Les cellules souches : de leurs niches à leurs applications thérapeutiques, Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris-Sud - Paris 11 (UP11), Laboratoire de Biologie du Développement (LBD), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut de Biologie Paris Seine (IBPS), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), and Université Paris-Sud - Paris 11 (UP11)-Institut National de la Santé et de la Recherche Médicale (INSERM)
- Subjects
0301 basic medicine ,Time Factors ,Apoptosis ,Regenerative medicine ,Mice ,[SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB] ,Stem Cell Niche ,Research Articles ,ComputingMilieux_MISCELLANEOUS ,Gene Expression Regulation, Developmental ,[SDV.BBM.MN]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular Networks [q-bio.MN] ,[SDV.MHEP.HEM]Life Sciences [q-bio]/Human health and pathology/Hematology ,3. Good health ,Cell biology ,Haematopoiesis ,Phenotype ,Liver ,Female ,Stem cell ,Signal Transduction ,Genetic Markers ,Stromal cell ,Cell Survival ,Paracrine Communication ,[SDV.CAN]Life Sciences [q-bio]/Cancer ,Biology ,Transfection ,Article ,Cell Line ,Extracellular Vesicles ,03 medical and health sciences ,[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN] ,Animals ,RNA, Messenger ,Progenitor cell ,Gene Expression Profiling ,Mesenchymal stem cell ,Cell Biology ,Hematopoietic Stem Cells ,Coculture Techniques ,Mice, Inbred C57BL ,MicroRNAs ,[SDV.BDD.EO]Life Sciences [q-bio]/Development Biology/Embryology and Organogenesis ,030104 developmental biology ,Stromal Cells ,Transcriptome ,Ex vivo - Abstract
Extracellular vesicles (EVs) are emerging as crucial mediators in cell-to-cell communication. Stik et al. provide evidence that EVs released by supportive stromal cells target hematopoietic stem and progenitor cells in vivo and in vitro and influence their gene expression and potential., Extracellular vesicles (EVs) have been recently reported as crucial mediators in cell-to-cell communication in development and disease. In this study, we investigate whether mesenchymal stromal cells that constitute a supportive microenvironment for hematopoietic stem and progenitor cells (HSPCs) released EVs that could affect the gene expression and function of HSPCs. By taking advantage of two fetal liver–derived stromal lines with widely differing abilities to maintain HSPCs ex vivo, we demonstrate that stromal EVs play a critical role in the regulation of HSPCs. Both supportive and nonsupportive stromal lines secreted EVs, but only those delivered by the supportive line were taken up by HSPCs ex vivo and in vivo. These EVs harbored a specific molecular signature, modulated the gene expression in HSPCs after uptake, and maintained the survival and clonogenic potential of HSPCs, presumably by preventing apoptosis. In conclusion, our study reveals that EVs are an important component of the HSPC niche, which may have major applications in regenerative medicine.
- Published
- 2017
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