1. BRG1 promotes the repair of DNA double-strand breaks by facilitating the replacement of RPA with RAD51
- Author
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Wenjing, Qi, Ruoxi, Wang, Hongyu, Chen, Xiaolin, Wang, Ting, Xiao, Istvan, Boldogh, Xueqing, Ba, Liping, Han, and Xianlu, Zeng
- Subjects
DNA End-Joining Repair ,fungi ,genetic processes ,DNA Helicases ,DNA, Single-Stranded ,Nuclear Proteins ,Chromatin Assembly and Disassembly ,Genomic Instability ,Cell Line ,Rad52 DNA Repair and Recombination Protein ,enzymes and coenzymes (carbohydrates) ,Replication Protein A ,health occupations ,Humans ,DNA Breaks, Double-Stranded ,Rad51 Recombinase ,biological phenomena, cell phenomena, and immunity ,Homologous Recombination ,DNA Damage ,Etoposide ,Transcription Factors ,Research Article - Abstract
DNA double-strand breaks (DSBs) are a type of lethal DNA damage. The repair of DSBs requires tight coordination between the factors modulating chromatin structure and the DNA repair machinery. BRG1, the ATPase subunit of the chromatin remodelling complex Switch/Sucrose non-fermentable (SWI/SNF), is often linked to tumorigenesis and genome instability, and its role in DSB repair remains largely unclear. In the present study, we show that BRG1 is recruited to DSB sites and enhances DSB repair. Using DR-GFP and EJ5-GFP reporter systems, we demonstrate that BRG1 facilitates homologous recombination repair rather than nonhomologous end-joining (NHEJ) repair. Moreover, the BRG1–RAD52 complex mediates the replacement of RPA with RAD51 on single-stranded DNA (ssDNA) to initiate DNA strand invasion. Loss of BRG1 results in a failure of RAD51 loading onto ssDNA, abnormal homologous recombination repair and enhanced DSB-induced lethality. Our present study provides a mechanistic insight into how BRG1, which is known to be involved in chromatin remodelling, plays a substantial role in the homologous recombination repair pathway in mammalian cells.
- Published
- 2014