1. Euphorbia factor L1 reverses ABCB1-mediated multidrug resistance involving interaction with ABCB1 independent of ABCB1 downregualtion
- Author
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Yan Jun Mi, Cheng Jun Shi, Shu Peng Chen, Jian Ye Zhang, Li Yang Tao, Li Ming Chen, Yong Ju Liang, Li Sheng Zheng, Hubiao Chen, Li Wu Fu, and Fang Wang
- Subjects
ATP Binding Cassette Transporter, Subfamily B ,Cell Survival ,Blotting, Western ,Down-Regulation ,ATP-binding cassette transporter ,Biology ,Pharmacology ,Biochemistry ,Rhodamine 123 ,chemistry.chemical_compound ,Adenosine Triphosphate ,Downregulation and upregulation ,Euphorbia ,Cell Line, Tumor ,medicine ,Humans ,Doxorubicin ,ATP Binding Cassette Transporter, Subfamily B, Member 1 ,skin and connective tissue diseases ,Molecular Biology ,Adenosine Triphosphatases ,Antibiotics, Antineoplastic ,Dose-Response Relationship, Drug ,Molecular Structure ,Phenylpropionates ,Plant Extracts ,Reverse Transcriptase Polymerase Chain Reaction ,Hydrolysis ,Cell Biology ,Flow Cytometry ,biology.organism_classification ,Drug Resistance, Multiple ,Multiple drug resistance ,chemistry ,Drug Resistance, Neoplasm ,Cell culture ,Efflux ,Diterpenes ,medicine.drug - Abstract
Euphorbia factor L1 (EFL1) belongs to diterpenoids of genus Euphorbia. In this article, its reversal activity against ABCB1-mediated MDR in KBv200 and MCF-7/adr cells was reported. However, EFL1 did not alter the sensitivity of KB and MCF-7 cells to chemotherapeutic agents. Meanwhile, EFL1 significantly increased accumulation of doxorubicin and rhodamine 123 in KBv200 and MCF-7/adr cells, showing no significant influence on that of KB and MCF-7 cells. Furthermore, EFL1 could enhance the ATP hydrolysis activity of ABCB1 stimulated by verapamil. At the same time, EFL1 inhibited the efflux of ABCB1 in KBv200 and MCF-7/adr cells. In addition, EFL1 did not downregulate expression of ABCB1 in KBv200 and MCF-7/adr cells either in mRNA or protein level.
- Published
- 2011
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