1. Adenylyl cyclase activity and gene expression during mesodermal differentiation of the P19 embryonal carcinoma cells.
- Author
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Lipskaia L, Grépin C, Defer N, and Hanoune J
- Subjects
- Adenylyl Cyclases genetics, Animals, Calcium pharmacology, Calcium-Calmodulin-Dependent Protein Kinases antagonists & inhibitors, Calmodulin pharmacology, Colforsin pharmacology, Cyclic AMP metabolism, DNA-Binding Proteins genetics, Dimethyl Sulfoxide pharmacology, GATA4 Transcription Factor, Isoenzymes genetics, Isoenzymes metabolism, Keratins genetics, Mice, Myosin Light Chains physiology, RNA, Messenger metabolism, Sulfonamides pharmacology, Transcription Factors genetics, Tumor Cells, Cultured, Adenylyl Cyclases metabolism, Cell Differentiation physiology, Gene Expression Regulation, Developmental genetics, Heart embryology
- Abstract
DMSO-primed P19 pluripotent cells, which recapitulate the first stages of mammalian cardiogenesis and endodermal formation, were used as an in vitro model to analyze the variations in activity and expression of the different adenylyl cyclase (AC) isoforms during the early events of embryonic cell differentiation. Here, we show that the total AC activity, which increases up to 10-fold after differentiation of P19 cells, is mainly associated with increases in AC2, AC5, and AC6 mRNA levels. Particularly, the marked increase in AC5 mRNA correlates with the appearance of beating cardiomyocytes and with the transcription of the atrial myosin light chain (MLC1A) gene which encodes a protein specifically involved in the cardiac muscle cell contractile phenotype. Together, the results strongly suggest that 1) a rise in cyclic AMP (cAMP) may be associated with cardiomyocyte and endodermal cell differentiation during mammalian embryogenesis; and 2) AC5 gene expression starts very early during normal mouse cardiogenesis and correlates with the differentiation of cardiomyocytes.
- Published
- 1998
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