1. Transplantation of mesenchymal stem cells overexpressing IL10 attenuates cardiac impairments in rats with myocardial infarction
- Author
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Xin Meng, Jianping Li, Hongliang Liang, Jinzhou Zhang, Liwen Liu, Ming Yu, Minjuan Zheng, Jian Yang, and Chao Sun
- Subjects
0301 basic medicine ,Time Factors ,Physiology ,Clinical Biochemistry ,Myocardial Infarction ,Apoptosis ,030204 cardiovascular system & hematology ,Pharmacology ,Ventricular Function, Left ,Rats, Sprague-Dawley ,0302 clinical medicine ,immune system diseases ,Transduction, Genetic ,Myocardial infarction ,Cells, Cultured ,hemic and immune systems ,Cell Hypoxia ,Interleukin-10 ,Up-Regulation ,Interleukin 10 ,medicine.symptom ,musculoskeletal diseases ,Cell Survival ,Genetic Vectors ,chemical and pharmacologic phenomena ,Inflammation ,Mesenchymal Stem Cell Transplantation ,Transfection ,Adenoviridae ,03 medical and health sciences ,parasitic diseases ,medicine ,Ventricular Pressure ,Animals ,Secretion ,Viability assay ,business.industry ,Myocardium ,Mesenchymal stem cell ,Mesenchymal Stem Cells ,Cell Biology ,Genetic Therapy ,medicine.disease ,Myocardial Contraction ,Transplantation ,Disease Models, Animal ,030104 developmental biology ,Glucose ,Immunology ,business - Abstract
Mesenchymal stem cell (MSC) has been well known to exert therapeutic potential for patients with myocardial infarction (MI). In addition, interleukin-10 (IL10) could attenuate MI through suppressing inflammation. Thus, the combination of MSC implantation with IL10 delivery may extend health benefits to ameliorate cardiac injury after MI. Here we established overexpression of IL10 in bone marrow-derived MSC through adenoviral transduction. Cell viability, apoptosis, and IL10 secretion under ischemic challenge in vitro were examined. In addition, MSC was transplanted into the injured hearts in a rat model of MI. Four weeks after the MI induction, MI, cardiac functions, apoptotic cells, and inflammation cytokines were assessed. In response to in vitro oxygen-glucose deprivation (OGD), IL10 overexpression in MSC (Ad.IL10-MSC) enhanced cell viability, decreased apoptosis, and increased IL10 secretion. Consistently, the implantation of Ad.IL10-MSCs into MI animals resulted in more reductions in myocardial infarct size, cardiac impairment, and cell apoptosis, compared to the individual treatments of either MSC or IL10 administration. Moreover, the attenuation of both systemic and local inflammations was most prominent for Ad.IL10-MSC treatment. IL10 overexpression and MSC may exert a synergistic anti-inflammatory effect to alleviate cardiac injury after MI.
- Published
- 2017