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This paper primarily aims to address aspects related to regional development in Romania, which has become a challenge both at the national and global level. The way regional development is approached is different, depending on the particularities of each country, as well as the governing ideologies in each state. However, the goal is common, one aims at shaping a competitive and strong economy, a high standard of living, access to education and medical assistance. The purpose of the paper is to compare socio-economic indicators from two time periods, to highlight similarities and differences and to observe their evolution within a 10-year timespan. The method used is Principal Component Analysis (PCA) to identify groups of statistical indicators (variables) that explain the level of development within a sample, in our case the development regions of Romania. In the context of regional development, PCA can be applied to extract relevant information about economic, social or demographic variability in different regions of the country. Several objectives of regional development analyzed through the lens of this method were pursued: identification of regional disparities, optimization of resource allocation, evaluation of the determining factors of economic development, monitoring of the impact of regional policies. Following the PCA analysis, it was observed that regional development cannot be evaluated by means of variables belonging to a single sector. This method allowed the identification of the variables that have the greatest weight describing economic, social, educational and infrastructure aspects. PCA was applied for two time periods and similar situations were obtained, the first main component called by the authors the economic statistics includes for both periods the indicators: Gross average nominal salary gain, GDP by development regions, Employees from research and development activity at the end of the year, Beds in health facilities (state and private). The second component was called social statistics and includes the indicator Employment rate in working age (15-64 years), and the third component called agricultural statistics includes the indicator Population served by the public water supply system. As a conclusion, we consider that the situations are not overlapping because there are indicators that have undergone changes during the 10 years, which is expected and normal in regional development. For example, the number of graduates decreased due to the continuously decreasing birth rate, the vacancy rate decreased in 2018-2019 compared to 2008-2009 because Romania went through the financial economic crisis.

The paper traces how the light source and light color influence the regenerative processes and morphogenesis in vitro cultures of Rebutia heliosa. To establish the vitroculture of R. heliosa, some explants from young plants, either buds or rounds cut from young stems and cross-sectioned were considered. The culture medium consisted of: macroelements and Fe EDTA Murashige-Skoog (1962), microelements Heller (1953), a mineral mixture to which the following vitamins were added: pyridoxine HCl, thiamine HCl and nicotinic acid (each 1 mg/l, each), m-inositol – 100 mg/l, sucrose – 20 g/l and agar-agar 7 g/l, without growth phytoregulators.The variable element was the lighting source. Fluorescent tubes and LEDs (monochrome but differently coloured) have been used: blue (λ = 470 nm), yellow (λ = 580 nm), green (λ = 540 nm), red (λ = 670 nm) as well as LED`s emitting white light (λ = 510 nm) at a luminous intensity of 1000 lux. The evolution of R. heliosa vitro cultures was monitored for 90 days and analyzed their reaction variability. Based on the comparative study, the following can be stated: the light of fluorescent tubes is more suitable for the morphogenesis of R. heliosa vitroplants, but the processes of regeneration and organogenesis are directly influenced by the source and color of light influenced, thus rhizogenesis and caulogenesis is favored by the presence of green and red light emitted by LEDs, while the white and yellow light of fluorescent tubes favors the caulogenesis and callusogenesis processes.

This paper delves into a thorough risk assessment of glyphosate, a widely used herbicide, and its metabolite, aminomethylphosphonic acid (AMPA), within the aquatic ecosystems of the Danube-Tisa-Danube (DTD) network, which represents large land drained system between Serbia, Hungary, and Romania. The primary objective is to employ a Toxicity and Exposure Ratio (TER) framework, encompassing both acute and chronic considerations, to comprehensively evaluate the potential risks these agrochemicals pose to aquatic organisms in the intricate hydro system of the DTD. The study integrates field data, laboratory experiments, and mathematical modeling to quantify glyphosate and AMPA exposure levels in the DTD channels. Furthermore, it explores the toxicological impacts of these substances on a diverse range of aquatic organisms, such as fish, invertebrates, and amphibians, considering acute effects arising from short-term exposure and chronic effects resulting from prolonged interactions. This research aims to provide a holistic understanding of the risk landscape associated with glyphosate and AMPA in the DTD hydro system by employing the TER approach. The findings contribute valuable insights into the potential ecological implications of herbicide usage in this critical water network, aiding regulatory decision-making and facilitating the development of targeted mitigation strategies to safeguard aquatic biodiversity and ecosystem health.