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The therapeutic monitoring of flecainide, like the other antiarrhythmic agents, has become an integral part of the administration of this type of drug. A full description is given here of a high-performance liquid chromatographic method which has been developed and used over the past three years for the routine monitoring of flecainide. This method uses extraction into toluene using the non-fluorinated analogue of flecainide as an internal standard and then direct back-extraction into a small volume of aqueous acid without the need for solvent evaporation. A fluorescence detection instrument is chosen which avoids the use of high-cost fluorometers. Ample sensitivity is accomplished with a simple fluorometer by selecting a combination of Schott bandpass filters with high-transmittance characteristics at appropriate wavelengths. A comparison is made between this method and the newly introduced fluorescence polarisation immunoassay. The comparison shows a good agreement between the independent technologies.