1. Determination of phloroglucinol in human plasma by gas chromatography-mass spectrometry.
- Author
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Lartigue-Mattei C, Lauro-Marty C, Bastide M, Berger JA, Chabard JL, Goutay E, and Aiache JM
- Subjects
- Drug Stability, Drug Storage, Humans, Phloroglucinol pharmacokinetics, Reproducibility of Results, Gas Chromatography-Mass Spectrometry methods, Phloroglucinol blood
- Abstract
A specific and sensitive method has been developed for the determination of phloroglucinol in plasma; it involves an optimized procedure for blood sampling designed to minimize the in vitro oxidation of the molecule, and gas chromatography-mass spectrometry after silylation of the compound. The method allowed a reliable determination of phloroglucinol in plasma. The precision and accuracy of the assay, reported as coefficients of variation, were below 15%. Using a plasma sample of 0.25 ml, the limit of quantitation was 5 ng/ml with a precision of 17.4%, which is sensitive enough for pharmacokinetic studies. Stability studies under different conditions revealed that ascorbic acid limits the degradation of phloroglucinol in plasma during storage at freezer temperatures.
- Published
- 1993
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