1. Quantification of selected furocoumarins by high-performance liquid chromatography and UV-detection: Capabilities and limits
- Author
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Berthold Weber, Angus P. Macmaster, Nikola Bast, G. Loesing, Matthias Vey, Christine Schippa, Alan Sherlock, Alain Chaintreau, Neil Owen, Emeline Tissot, Hugues Brevard, Richard D. Hiserodt, Sylvain Brussaux, Eric Frérot, and Hans Leijs
- Subjects
Detection limit ,Chromatography ,medicine.diagnostic_test ,Chemistry ,Furocoumarin ,Organic Chemistry ,Reproducibility of Results ,General Medicine ,Complex Mixtures ,Sensitivity and Specificity ,Biochemistry ,High-performance liquid chromatography ,Spectral similarity ,Analytical Chemistry ,Furocoumarins ,Spectrophotometry ,Oils, Volatile ,medicine ,Spectrophotometry, Ultraviolet ,Uv detection ,Chromatography, High Pressure Liquid ,Reliability (statistics) - Abstract
The performance of HPLC-UV as a means of quantifying selected furocoumarins in essential oils has been evaluated, based on a ring test validation approach. Accuracy profiles were generated, to determine bias and statistical confidence associated with determination at different concentrations, along with lower limits of quantification (LOQ). From these findings, it can be concluded that the method described may only be used in simple cases (essential oils), to measure individual furocoumarin compounds at concentrations greater than 10mg/l; the non compound-specific nature of detection by absorption in the UV range is unable to overcome the effect of interferences arising from chromatographic coelutions, such as those encountered in the analysis of complex commercial fragrance mixtures. The use of an algorithmically calculated 'spectral similarity' function, with reference to authentic standards, may be used to improve reliability in assignment and quantification.
- Published
- 2012
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