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Start Over Author "Fu-Tian Yu" Journal journal of chromatography a
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1. A reliable method of high performance liquid chromatography coupled with inductively coupled plasma mass spectrometry for determining selenoamino acids in selenoproteins from Lactococcus lactis

Author

Jing Jing Peng, Yang Liu, Fu Tian Yu, He Liang Fan, Shi Yang Yue, Yu Hui Fang, Xiao Ling Liu, and Cheng-Hua Wang

Subjects
Lactococcus lactis, Selenium, Organic Chemistry, General Medicine, Selenoproteins, Biochemistry, Chromatography, High Pressure Liquid, Mass Spectrometry, Analytical Chemistry
Abstract

A reliable method for simultaneous determination of four organic selenium species by HPLC-ICP-MS was developed and implemented in determining organic selenoamino acids (Se-AAs) in selenoproteins from Lactococcus lactis (L. lactis) NZ9000. The method consisted of liberating Se-AAs from selenoproteins using ultrasound-assisted protease hydrolysis, and quantitatively detecting Se-AA speciations by HPLC-ICP-MS. After optimizations of proteolysis conditions, chromatographic conditions and determination conditions, the established method could efficiently separate the four Se-AAs, including SeCys, SeCys2, SeMeCys and SeMet within 10 min. It presented high sensitivity with the limits of detection and quantitation in the range of 0.197∼0.240 μg∙L

Published
2022

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