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Your search keyword '"Unger, K.K."' showing total 95 results
Start Over Author "Unger, K.K." Journal journal of chromatography a
95 results on '"Unger, K.K."'

29. Assessment and optimization of system parameters in size exclusion separation of proteins on diol-modified silica columns

Author

Roumeliotis, P. and Unger, K.K.

Abstract

On diol-modified silica columns the retention of proteins is governed by a size exclusion effect, but superimposed on this are some secondary effects, i.e., ionic and diol-ligand interactions which can be controlled and adjusted reproducibly by varying the eluent composition. The eluent composition also affects the column efficiency and peak shape. Both dependences can be employed to obtain a better resolution of proteins than can be expected from size exclusion alone.

Published
1981
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30. Comparative study of zorbax bio series GF 250 and GF 450 and TSK-Gel 3000 SW and SWXL columns in size-exclusion chromatography of proteins

Author

Anspach, B., Gierlich, H.U., and Unger, K.K.

Abstract

A reduction of the mean particle diameter of silica-based packings in the size-exclusion chromatography (SEC) of proteins to about 5 μm generates the expected increase in column plate number over the traditional 10 μm SEC columns, as demonstrated for the Zorbax Bio Series GF 250 and GF 450 and TSK-Gel 3000 SWXL columns. The slightly lower column efficiency of the TSK-Gel 3000 SWXL compared with the GF 250 column is compensated by the fact that the phase ratio of the 3000 SWXL column is higher by a factor of two. Hence both columns show nearly the same peak capacity of about 20–30. When the ionic strength of the eluent was changed by varying the salt concentration, the elution volume of proteins was found to be affected differently on the two columns. These alterations are attributed to the differences in the surface compositions of the two types of packings.

Published
1988
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