1. High-performance liquid chromatographic determination of iophenoxic acid in serum
- Author
-
Ainsley Jones
- Subjects
Detection limit ,Iophenoxic acid ,Chromatography ,Chemistry ,Foxes ,chemistry.chemical_element ,General Chemistry ,Iopanoic Acid ,Iodine ,High-performance liquid chromatography ,Biological fluid ,Biological fluids ,Animals ,Humans ,Spectrophotometry, Ultraviolet ,Quantitative analysis (chemistry) ,Chromatography, High Pressure Liquid ,Analysis method - Abstract
Iophenoxic acid (IPA), a marker used to investigate the feeding behaviour of bait-consuming animals has previously been indirectly determined by measuring protein-bound iodine levels in serum or plasma. For the first time a method is reported for the direct determination of IPA in biological fluids. IPA was determined in de-proteinized serum by high-performance liquid chromatography (HPLC) on a C18 column with a mobile phase of acetonitrile-water. Isocratic and gradient systems are described with limits of detection of 0.2 microgram/ml (isocratic) and 0.05 microgram/ml (gradient). Recoveries from fox serum were 85% at 0.5 microgram/ml, 95% at 5 micrograms/ml and 91% at 50 micrograms/ml.
- Published
- 1994
- Full Text
- View/download PDF