1. Rapid determination of some psychotropic drugs in complex matrices by tandem dispersive liquid-liquid microextraction followed by high performance liquid chromatography.
- Author
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Asghari A, Fahimi E, Bazregar M, Rajabi M, and Boutorabi L
- Subjects
- Chlorpromazine analysis, Chlorpromazine blood, Chlorpromazine isolation & purification, Chromatography, High Pressure Liquid economics, Clozapine analysis, Clozapine blood, Clozapine isolation & purification, Humans, Limit of Detection, Liquid Phase Microextraction economics, Psychotropic Drugs isolation & purification, Thioridazine analysis, Thioridazine blood, Thioridazine isolation & purification, Time Factors, Chromatography, High Pressure Liquid methods, Liquid Phase Microextraction methods, Psychotropic Drugs analysis, Psychotropic Drugs blood, Wastewater analysis
- Abstract
Simple and rapid determinations of some psychotropic drugs in some pharmaceutical wastewater and human plasma samples were successfully accomplished via the tandem dispersive liquid-liquid microextraction combined with high performance liquid chromatography-ultraviolet detection (TDLLME-HPLC-UV). TDLLME of the three psychotropic drugs clozapine, chlorpromazine, and thioridazine was easily performed through two consecutive dispersive liquid-liquid microextractions. By performing this convenient method, proper sample preconcentrations and clean-ups were achieved in just about 7min. In order to achieve the best extraction efficiency, the effective parameters involved were optimized. The optimal experimental conditions consisted of 100μL of CCl
4 (as the extraction organic solvent), and the pH values of 13 and 2 for the donor and acceptor phases, respectively. Under these optimum experimental conditions, the proposed TDLLME-HPLC-UV technique provided a good linearity in the range of 5-3000ngmL-1 for the three psychotropic drugs with the correlation of determinations (R2 s) higher than 0.996. The limits of quantification (LOQs) and limits of detection (LODs) obtained were 5.0ngmL-1 and 1.0-1.5ngmL-1 , respectively. Also the proper enrichment factors (EFs) of 96, 99, and 88 for clozapine, chlorpromazine, and thioridazine, respectively, and good extraction repeatabilities (relative standard deviations below 9.3%, n=5) were obtained., (Copyright © 2017 Elsevier B.V. All rights reserved.)- Published
- 2017
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