1. Cloning and expression of fatty acids biosynthesis key enzymes from sunflower (Helianthus annuus L.) in Escherichia coli.
- Author
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Serrano-Vega MJ, Venegas-Calerón M, Garcés R, and Martínez-Force E
- Subjects
- Amino Acid Sequence, Base Sequence, Cloning, Molecular, DNA Primers, Electrophoresis, Polyacrylamide Gel, Mixed Function Oxygenases isolation & purification, Mixed Function Oxygenases metabolism, Recombinant Proteins genetics, Recombinant Proteins isolation & purification, Recombinant Proteins metabolism, Seeds enzymology, Thiolester Hydrolases isolation & purification, Thiolester Hydrolases metabolism, Escherichia coli genetics, Fatty Acids biosynthesis, Helianthus enzymology, Mixed Function Oxygenases genetics, Thiolester Hydrolases genetics
- Abstract
To further characterize the stearoyl-acyl carrier protein (ACP) desaturase (EC 1.14.99.6) and the acyl-ACP thioesterase FatB (EC 3.1.2.14) activities from sunflower seeds, we cloned, sequenced and expressed the recombinant genes in Escherichia coli. We obtained two partially purified proteins, His-SAD and His-FATB, each of about 45000 Da. The expression of either proteins produced changes in the E. coli fatty acid profile indicating the functionality of the recombinant proteins. While the expression of His-SAD produced an effect similar to that produced by overexpression of the fabA gene, responsible for the fatty acid desaturation in E. coli, the expression of His-FATB gave rise to an unbalance between unsaturated fatty acids and a toxic effect in E. coli.
- Published
- 2003
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