1. Pd0 FOF1-ATPase prone pump modulated-detoxification of oxytetracycline in Bacillus megaterium Y-4.
- Author
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Yi, Linya, Chen, Shuyan, and Chen, Yuancai
- Subjects
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BACILLUS megaterium , *OXYTETRACYCLINE , *CHRONIC toxicity testing , *MICROBIAL enzymes , *STRUCTURAL equation modeling , *ENERGY consumption , *ELECTRON transport - Abstract
Bio-remediation are low-cost and environmentally friendly, but microbial enzymes tend to lose their activity when treated antibiotic contaminants due to its high toxicity. In this study, Bacillus megaterium Y-4 was demonstrated to in situ synthesize Pd0@cells, and detoxify oxytetracycline (OTC) through enhancing electron transport chain via Pd0-dependent F O F 1 -ATPase (proton pumps). Toxicity analyses showed that increasing Pd0 loading significantly reduced acute and chronic bio-toxicity as well as ecological risk of OTC effluent, as evidenced by the promoting cellular response to oxidative stress with increasing enzymes GSH-Px and IDH activities (4.24 and 1.20-fold). Electrochemical (I-t, LSV, DPV and EDC) showed that the incorporated bio-Pd0 mainly promoted FDH/Hase-based short chain (gradually took precedence over CoQ-based long chain), diverting OTC extracellular degradation to markedly detoxify OTC to cell. Inhibition (DCCD and TBA) assays and structural equation modelling indicated that bio-Pd0 upregulated ATP and NADH synthesis. It also acted as transmembrane F O F 1 -ATPase proton pump, which enabled the backward of protons out of the restriction of PMF, eventually maximizing the energy utilization. This study sheds new insights into the detoxification of antibiotics by bio-nanoparticles through proton transmembrane transfer/redistribution. [Display omitted] • OTC was extracellularly detoxified mainly through Pd0-mediated FDH/Hase-S chain. • Pd0 optimized cellular energy utilization through electron redistribution. • Pd0-dependent F O F 1 -ATPase acted as proton pump to enhance electron transfer. • Proton transfer was freed from PMF restriction. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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