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Understanding the driving mechanism of natural conditions and human activities on wetland ecosystem service value (WESV) change is important for the sustainable development of wetland resources. The existing studies often analyze the direct effects of natural conditions and human activities on WESV, however, the interaction mechanism of them on WESV is still unclear. Therefore, based on the remote sensing image data of Liaoning Province China in 1990–2020, this paper combined Emergy with Partial least squares-structural equation model (PLS-SEM) to evaluate main WESV and explore the interaction mechanism between socio-economic development factor (F sed), climate factor (F cl), environment factor (F en)and terrain factor (F te) on WESV change. The results showed that the provisioning (WESV P) and regulating (WESV R) services value increased by 6.93 E+14 and 3.01 E+14 solar emjoules (sej), respectively, while the cultural services value (WESV C) decreased by 1.50 E+14sej. Overall, the total value of wetland services value (WESV T) increased by 8.45 E+14sej during the study period, with a growth rate of 37.88%. F sed was the most significant factor driving WESV growth, with path coefficient of 0.53, followed by F en and F te , with path coefficient of 0.23 and 0.20, respectively, while F cl had the greatest negative effect on WESV, with path coefficient of −0.18. From the perspective of the interaction between each factor, F cl weakened the positive effect of F sed and F en on WESV, at the same time, F sed weakened the positive effect of F te on WESV. Through the indirect influence of F te , the adverse effect of F cl on WESV was further enhanced. This study innovatively revealed the interaction mechanism of natural conditions and human activities on WESV change, and the results could provide scientific basis for the management and sustainable development of wetland resources. [Display omitted] • Emergy theory was applied to calculate the main wetland ecosystem services value (WESV). • The interaction mechanism between natural conditions and human activities on WESV change was revealed by PLS-SEM. • F sed played an important role in the indirect effect of WESV change, and the interaction between F cl and F en exacerbated their negative effect on WESV change. [ABSTRACT FROM AUTHOR]