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Start Over Author "Bornet C" Journal journal of clinical microbiology
2 results on '"Bornet C"'

1. Most Enterobacter aerogenes strains in France belong to a prevalent clone.

Author

Bosi, C, Davin-Regli, A, Bornet, C, Mallea, M, Pages, J M, and Bollet, C

Abstract

The aim of this study was to determine the distribution in France of the Enterobacter aerogenes prevalent clone isolated in the hospitals of the Marseille area (A. Davin-Regli, D. Monnet, P. Saux, C. Bosi, R. Charrel, A. Barthelemy, and C. Bollet, J. Clin. Microbiol. 34:1474-1480, 1996). A total of 123 E. aerogenes isolates were collected from 23 hospital laboratories and analyzed by random amplification of polymorphic DNA and enterobacterial repetitive intergenic consensus-PCR to determine their epidemiological relatedness. Molecular typing revealed that 21 of the 23 laboratories had isolated this prevalent clone harboring the plasmid encoding for extended-spectrum beta-lactamase of the TEM-24 type. Most isolates were susceptible only to imipenem and gentamicin. Their dissemination seems to be clonal and was probably the result of the general use of broad-spectrum cephalosporins and quinolones. Four isolates showed an alteration of their outer membrane proteins, causing decrease of susceptibility to third-generation cephalosporins and imipenem and leading to the critical situation of having no alternative therapeutic. The large dissemination of the E. aerogenes prevalent clone probably results from its good adaptation to the antibiotics administered in France and the hospital environment, particularly in intensive care units.

Published
1999

2. Imipenem resistance of enterobacter aerogenes mediated by outer membrane permeability.

Author

Bornet C, Davin-Regli A, Bosi C, Pages JM, and Bollet C

Subjects
Bacterial Typing Techniques, Cell Membrane Permeability, Cephalosporins therapeutic use, Enterobacter isolation & purification, Fatal Outcome, France, Humans, Imipenem pharmacology, Male, Microbial Sensitivity Tests, Thienamycins pharmacology, Trachea microbiology, Urine microbiology, beta-Lactamases genetics, Cefpirome, Drug Resistance, Microbial, Enterobacter drug effects, Enterobacter genetics, Enterobacteriaceae Infections drug therapy, Imipenem therapeutic use, Thienamycins therapeutic use
Abstract

Multidrug-resistant Enterobacter aerogenes strains are increasingly isolated in Europe and especially in France. Treatment leads to imipenem resistance, because of a lack of porin. We studied the evolution of resistance in 29 strains isolated from four patients during their clinical course. These strains belonged to the prevalent epidemiological type observed in France in previous studies (C. Bosi, et al., J. Clin. Microbiol. 37:2165-2169, 1999; A. Davin-Regli et al., J. Clin. Microbiol. 34:1474-1480, 1996). They also harbored a TEM-24 extended-spectrum beta-lactamase-coding gene. Thirteen strains were susceptible to gentamicin and resistant to imipenem and cefepime. All of the patients showed E. aerogenes strains with this resistance after an imipenem treatment. One patient showed resistance to imipenem after a treatment with cefpirome. Twelve of these 13 strains showed a lack of porin. Cessation of treatment with imipenem for three patients was followed by reversion of susceptibility to this antibiotic and the reappearance of porins, except in one case. For one patient, we observed three times in the same day the coexistence of resistant strains lacking porin and susceptible strains possessing porin. The emergence of multidrug-resistant E. aerogenes strains is very disquieting. In our study, infection by E. aerogenes increased the severity of the patients' illnesses, causing a 100% fatality rate.

Published
2000
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