Search

Your search keyword '"Donnelly, J Peter"' showing total 16 results
Start Over Author "Donnelly, J Peter" Journal journal of clinical microbiology
16 results on '"Donnelly, J Peter"'

1. Comparison of Nonculture Blood-Based Tests for Diagnosing Invasive Aspergillosis in an Animal Model

Author

White, P. Lewis, primary, Wiederhold, Nathan P., additional, Loeffler, Juergen, additional, Najvar, Laura K., additional, Melchers, Willem, additional, Herrera, Monica, additional, Bretagne, Stephane, additional, Wickes, Brian, additional, Kirkpatrick, William R., additional, Barnes, Rosemary A., additional, Donnelly, J. Peter, additional, and Patterson, Thomas F., additional

Published
2016
Full Text
View/download PDF

2. Analytical Comparison of In Vitro -Spiked Human Serum and Plasma for PCR-Based Detection of Aspergillus fumigatus DNA: a Study by the European Aspergillus PCR Initiative

Author

Loeffler, Juergen, primary, Mengoli, Carlo, additional, Springer, Jan, additional, Bretagne, Stéphane, additional, Cuenca-Estrella, Manuel, additional, Klingspor, Lena, additional, Lagrou, Katrien, additional, Melchers, Willem J. G., additional, Morton, C. Oliver, additional, Barnes, Rosemary A., additional, Donnelly, J. Peter, additional, and White, P. Lewis, additional

Published
2015
Full Text
View/download PDF

3. Clinical Performance of Aspergillus PCR for Testing Serum and Plasma: a Study by the European Aspergillus PCR Initiative

Author

White, P. Lewis, primary, Barnes, Rosemary A., additional, Springer, Jan, additional, Klingspor, Lena, additional, Cuenca-Estrella, Manuel, additional, Morton, C. Oliver, additional, Lagrou, Katrien, additional, Bretagne, Stéphane, additional, Melchers, Willem J. G., additional, Mengoli, Carlo, additional, Donnelly, J. Peter, additional, Heinz, Werner J., additional, and Loeffler, Juergen, additional

Published
2015
Full Text
View/download PDF

4. Evaluation of Aspergillus PCR Protocols for Testing Serum Specimens

Author

White, P. Lewis, primary, Mengoli, Carlo, additional, Bretagne, Stéphane, additional, Cuenca-Estrella, Manuel, additional, Finnstrom, Niklas, additional, Klingspor, Lena, additional, Melchers, Willem J. G., additional, McCulloch, Elaine, additional, Barnes, Rosemary A., additional, Donnelly, J. Peter, additional, and Loeffler, Juergen, additional

Published
2011
Full Text
View/download PDF

6. Aspergillus PCR: One Step Closer to Standardization

Author

White, P. Lewis, primary, Bretagne, Stéphane, additional, Klingspor, Lena, additional, Melchers, Willem J. G., additional, McCulloch, Elaine, additional, Schulz, Bettina, additional, Finnstrom, Niklas, additional, Mengoli, Carlo, additional, Barnes, Rosemary A., additional, Donnelly, J. Peter, additional, and Loeffler, Juergen, additional

Published
2010
Full Text
View/download PDF

8. Statistical Analyses of Correlation between Fluconazole MICs for Candida spp. Assessed by Standard Methods Set Forth by the European Committee on Antimicrobial Susceptibility Testing (E.Dis. 7.1) and CLSI (M27-A2)

Author

Rodriguez-Tudela, Juan Luis, primary, Donnelly, J. Peter, additional, Pfaller, Michael A., additional, Chryssantou, Erja, additional, Warn, Peter, additional, Denning, David W., additional, Espinel-Ingroff, Ana, additional, Barchiesi, Francesco, additional, and Cuenca-Estrella, Manuel, additional

Published
2007
Full Text
View/download PDF

12. Improved Detection of Circulating AspergillusAntigen by Use of a Modified Pretreatment Procedure

Author

Mennink-Kersten, Monique A. S. H., Ruegebrink, Dorien, Klont, Rocus R., Warris, Adilia, Blijlevens, Nicole M. A., Donnelly, J. Peter, and Verweij, Paul E.

Abstract

ABSTRACTDetection of circulating galactofuranose (galf) antigens, including galactomannan (GM), by the Platelia Aspergillus(PA) enzyme-linked immunosorbent assay (ELISA) is an important tool in the early diagnosis of invasive aspergillosis (IA). We used a modified pretreatment technique (MT) on consecutive negative PA ELISA plasma samples from IA patients in order to improve the detection of the fungal components present. Plasma samples (52) were collected from healthy donors, and 174 plasma samples with a galactomannan index (GMI) below 0.5 were collected from 25 unclassifiable and 23 IA patients. The PA ELISA reactivity of pretreated samples was determined before (conventional technique [CT]) and after (MT) filtration using a Microcon filter with a 50-kDa cutoff (Millipore). For the MT, the sensitivity of the PA ELISA increased from 42.9% (CT) to 78.6% (MT) using a cutoff for the GMI of 1.5 in the probable and proven group, whereas specificity slightly decreased from 98.7% to 96.1% in the control group. The 10-fold concentration step increased the GMI as high as 121-fold. The MT resulted not only in positive reactivity in samples that tested negative with the CT but also in the earlier detection of antigen by 2 to 17 days.

Published
2008
Full Text
View/download PDF

13. Colorimetric Assay for Antifungal Susceptibility Testing ofAspergillusSpecies

Author

Meletiadis, Joseph, Mouton, Johan W., Meis, Jacques F. G. M., Bouman, Bianca A., Donnelly, J. Peter, and Verweij, Paul E.

Abstract

ABSTRACTA colorimetric assay for antifungal susceptibility testing ofAspergillusspecies (Aspergillus fumigatus,Aspergillus flavus,Aspergillus terreus,Aspergillus nidulans, and Aspergillus ustus) is described based on the reduction of the tetrazolium salt 2,3-bis(2-methoxy-4-nitro-5-[(sulphenylamino)carbonyl]-2H-tetrazolium-hydroxide (XTT) in the presence of menadione as an electron-coupling agent. The combination of 200 µg of XTT/ml with 25 µM menadione resulted in a high production of formazan within 2 h of exposure, allowing the detection of hyphae formed by low inocula of 102CFU/ml after 24 h of incubation. Under these settings, the formazan production correlated linearly with the fungal biomass and less-variable concentration effect curves for amphotericin B and itraconazole were obtained.

Published
2001
Full Text
View/download PDF

14. Development and Evaluation of a Calibrator Material for Nucleic Acid-Based Assays for Diagnosing Aspergillosis

Author

Lyon, G. Marshall, Abdul-Ali, Deborah, Loeffler, Juergen, White, P. Lewis, Wickes, Brian, Herrera, Monica L., Alexander, Barbara D., Baden, Lindsey R., Clancy, Cornelius, Denning, David, Nguyen, M. Hong, Sugrue, Michele, Wheat, L. Joseph, Wingard, John R., Donnelly, J. Peter, Barnes, Rosemary, Patterson, Thomas F., and Caliendo, Angela M.

Abstract

ABSTRACTTwelve laboratories evaluated candidate material for an AspergillusDNA calibrator. The DNA material was quantified using limiting-dilution analysis; the mean concentration was determined to be 1.73 × 1010units/ml. The calibrator can be used to standardize aspergillosis diagnostic assays which detect and/or quantify nucleic acid.

Published
2013
Full Text
View/download PDF

15. Critical Stages of Extracting DNA from Aspergillus fumigatusin Whole-Blood Specimens

Author

White, P. Lewis, Perry, Michael D., Loeffler, Juergen, Melchers, Willem, Klingspor, Lena, Bretagne, Stephane, McCulloch, Elaine, Cuenca-Estrella, Manuel, Finnstrom, Niklas, Donnelly, J. Peter, and Barnes, Rosemary A.

Abstract

ABSTRACTA standardized protocol for extracting DNA from Aspergillus fumigatushas been proposed by the European AspergillusPCR Initiative (EAPCRI). Using meta-regression analysis, the EAPCRI showed certain stages of the process to be critical to providing a satisfactory analytical sensitivity. The study investigated each step of the EAPCRI protocol by elimination and monitored the influence on AspergillusPCR performance.

Published
2010
Full Text
View/download PDF

16. Statistical Analyses of Correlation between Fluconazole MICs for Candidaspp. Assessed by Standard Methods Set Forth by the European Committee on Antimicrobial Susceptibility Testing (E.Dis. 7.1) and CLSI (M27-A2)

Author

Rodriguez-Tudela, Juan Luis, Donnelly, J. Peter, Pfaller, Michael A., Chryssantou, Erja, Warn, Peter, Denning, David W., Espinel-Ingroff, Ana, Barchiesi, Francesco, and Cuenca-Estrella, Manuel

Abstract

ABSTRACTThe European Committee on Antimicrobial Susceptibility Testing (EUCAST) Subcommittee on Antifungal Susceptibility Testing recently published a standard for determining the susceptibility of fermentative yeasts to antifungals. From the beginning, the EUCAST and its North American counterpart, the CLSI, decided to work together in order to establish common standards. As part of this exercise, the susceptibility of a set of 475 yeast isolates was tested by both standards. The intraclass correlation coefficient and the equations defining the linear regression between both methods were estimated. Both methods produced very similar results, with an intraclass correlation coefficient of 0.954 (0.945 to 0.962), although linear regression analysis shows that the EUCAST standard resulted in slightly lower MICs. There were only eight isolates showing at least four twofold dilution MIC differences between both standards. After 24 h of incubation, the MICs obtained by the CLSI method were equivalent to those obtained by the EUCAST standard. In summary, both methods produce very similar MICs, indicating that methodology does not pose any obstacle to obtaining uniform standards for antifungal susceptibility testing of yeasts.

Published
2007
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results