1. Species Identification of Clinical Prevotella Isolates by Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry
- Author
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Oriane Soetens, Fedoua Echahidi, Denis Pierard, Annelies De Bel, Ingrid Wybo, Ellen Vancutsem, Kristof Vandoorslaer, Immunology and Microbiology, Microbiology and Infection Control, and Clinical Biology
- Subjects
Microbiology (medical) ,Chromatography ,Sequence Analysis, RNA ,Molecular Sequence Data ,Prevotella ,Bacteriology ,Matrix assisted laser desorption ionization time of flight ,Reference Standards ,Biology ,Mass spectrometry ,biology.organism_classification ,Molecular biology ,RNA, Bacterial ,Species Specificity ,Species level ,RNA, Ribosomal, 16S ,Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ,16s rrna gene sequencing ,Species identification ,Time-of-flight mass spectrometry - Abstract
The performance of matrix-assisted laser desorption–ionization time of flight mass spectrometry (MALDI-TOF MS) for species identification of Prevotella was evaluated and compared with 16S rRNA gene sequencing. Using a Bruker database, 62.7% of the 102 clinical isolates were identified to the species level and 73.5% to the genus level. Extension of the commercial database improved these figures to, respectively, 83.3% and 89.2%. MALDI-TOF MS identification of Prevotella is reliable but needs a more extensive database.
- Published
- 2012