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Your search keyword '"Limmathurotsakul, Direk"' showing total 21 results
Start Over Author "Limmathurotsakul, Direk" Journal journal of clinical microbiology
21 results on '"Limmathurotsakul, Direk"'

1. Viruses in Vietnamese Patients Presenting with Community-Acquired Sepsis of Unknown Cause

Author

Anh, Nguyen To, primary, Hong, Nguyen Thi Thu, additional, Nhu, Le Nguyen Truc, additional, Thanh, Tran Tan, additional, Lau, Chuen-Yen, additional, Limmathurotsakul, Direk, additional, Deng, Xutao, additional, Rahman, Motiur, additional, Chau, Nguyen Van Vinh, additional, van Doorn, H. Rogier, additional, Thwaites, Guy, additional, Delwart, Eric, additional, and Tan, Le Van, additional

Published
2019
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2. A Rapid Immunochromatography Test Based on Hcp1 Is a Potential Point-of-Care Test for Serological Diagnosis of Melioidosis

Author

Phokrai, Phornpun, primary, Karoonboonyanan, Wisansanee, additional, Thanapattarapairoj, Nida, additional, Promkong, Chidchanok, additional, Dulsuk, Adul, additional, Koosakulnirand, Sirikamon, additional, Canovali, Sasha, additional, Indrawattana, Nitaya, additional, Jutrakul, Yaowaruk, additional, Wuthiekanun, Vanaporn, additional, Limmathurotsakul, Direk, additional, Brett, Paul J., additional, Burtnick, Mary N., additional, Lertmemongkolchai, Ganjana, additional, and Chantratita, Narisara, additional

Published
2018
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4. Optimal Cutoff and Accuracy of an IgM Enzyme-Linked Immunosorbent Assay for Diagnosis of Acute Scrub Typhus in Northern Thailand: an Alternative Reference Method to the IgM Immunofluorescence Assay

Author

Blacksell, Stuart D., primary, Lim, Cherry, additional, Tanganuchitcharnchai, Ampai, additional, Jintaworn, Suthatip, additional, Kantipong, Pacharee, additional, Richards, Allen L., additional, Paris, Daniel H., additional, Limmathurotsakul, Direk, additional, and Day, Nicholas P. J., additional

Published
2016
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12. Optimization of Culture of Leptospira from Humans with Leptospirosis

Author

Wuthiekanun, Vanaporn, primary, Chierakul, Wirongrong, additional, Limmathurotsakul, Direk, additional, Smythe, Lee D., additional, Symonds, Meegan L., additional, Dohnt, Michael F., additional, Slack, Andrew T., additional, Limpaiboon, Roongreung, additional, Suputtamongkol, Yupin, additional, White, Nicholas J., additional, Day, Nicholas P. J., additional, and Peacock, Sharon J., additional

Published
2007
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13. Antibodies from Patients with Melioidosis Recognize Burkholderia mallei but Not Burkholderia thailandensis Antigens in the Indirect Hemagglutination Assay

Author

Tiyawisutsri, Rachaneeporn, primary, Peacock, Sharon J., additional, Langa, Sayan, additional, Limmathurotsakul, Direk, additional, Cheng, Allen C., additional, Chierakul, Wirongrong, additional, Chaowagul, Wipada, additional, Day, Nicholas P. J., additional, and Wuthiekanun, Vanaporn, additional

Published
2005
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15. Clinical and Molecular Epidemiology of Staphylococcus argenteusInfections in Thailand

Author

Thaipadungpanit, Janjira, Amornchai, Premjit, Nickerson, Emma K., Wongsuvan, Gumphol, Wuthiekanun, Vanaporn, Limmathurotsakul, Direk, and Peacock, Sharon J.

Abstract

ABSTRACTMolecular typing of 246 Staphylococcus aureusisolates from unselected patients in Thailand showed that 10 (4.1%) were actually Staphylococcus argenteus. Contrary to the suggestion that S. argenteusis less virulent than S. aureus, we demonstrated comparable rates of morbidity, death, and health care-associated infection in patients infected with either of these two species.

Published
2014
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16. Microevolution of Burkholderia pseudomalleiduring an Acute Infection

Author

Limmathurotsakul, Direk, Holden, Matthew T. G., Coupland, Paul, Price, Erin P., Chantratita, Narisara, Wuthiekanun, Vanaporn, Amornchai, Premjit, Parkhill, Julian, and Peacock, Sharon J.

Abstract

ABSTRACTWe used whole-genome sequencing to evaluate 69 independent colonies of Burkholderia pseudomalleiisolated from seven body sites of a patient with acute disseminated melioidosis. Fourteen closely related genotypes were found, providing evidence for the rapid in vivodiversification of B. pseudomalleiafter inoculation and systemic spread.

Published
2014
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17. Loop-Mediated Isothermal Amplification Method Targeting the TTS1 Gene Cluster for Detection of Burkholderia pseudomalleiand Diagnosis of Melioidosis

Author

Chantratita, Narisara, Meumann, Ella, Thanwisai, Aunchalee, Limmathurotsakul, Direk, Wuthiekanun, Vanaporn, Wannapasni, Saran, Tumapa, Sarinna, Day, Nicholas P. J., and Peacock, Sharon J.

Abstract

ABSTRACTMelioidosis is a severe infection caused by Burkholderia pseudomallei. The timely implementation of effective antimicrobial treatment requires rapid diagnosis. Loop-mediated isothermal amplification (LAMP) targeting the TTS1 gene cluster was developed for the detection of B. pseudomallei. LAMP was sensitive and specific for the laboratory detection of this organism. The lower limit of detection was 38 genomic copies per reaction, and LAMP was positive for 10 clinical B. pseudomalleiisolates but negative for 5 B. thailandensisand 5 B. malleiisolates. A clinical evaluation was conducted in northeast Thailand to compare LAMP to an established real-time PCR assay targeting the same TTS1 gene cluster. A total of 846 samples were obtained from 383 patients with suspected melioidosis, 77 of whom were subsequently diagnosed with culture-confirmed melioidosis. Of these 77 patients, a positive result was obtained from one or more specimens by PCR in 26 cases (sensitivity, 34%; 95% confidence interval [CI], 23.4 to 45.4%) and by LAMP in 34 cases (sensitivity, 44%; 95% CI, 32.8 to 55.9%) (P= 0.02). All samples from 306 patients that were culture negative for B. pseudomalleiwere negative by PCR (specificity, 100%; 95% CI, 98.8 to 100%), but 5 of 306 patients (1.6%) were positive by LAMP (specificity, 98.4%; 95% CI, 96.2 to 99.5%) (P= 0.03). The diagnostic accuracies of PCR and LAMP were 86.7% (95% CI, 82.9 to 89.9%) and 87.5% (95% CI, 83.7 to 90.6%), respectively (P= 0.47). Both assays were very insensitive when applied to blood samples; PCR and LAMP were positive for 0 and 1 of 44 positive blood cultures, respectively. The PCR and LAMP assays evaluated here are not sufficiently sensitive to replace culture in our clinical setting.

Published
2008
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18. Maintenance of LeptospiraSpecies in LeptospiraVanaporn Wuthiekanun Agar

Author

Wuthiekanun, Vanaporn, Amornchai, Premjit, Langla, Sayan, Oyuchua, Malinee, Day, Nicholas P. J., and Limmathurotsakul, Direk

Abstract

ABSTRACTThe maintenance of Leptospiraspecies in liquid or semisolid medium is time-consuming and at risk of contamination due to the needs of routine subculture and dark field microscopy. Using LeptospiraVanaporn Wuthiekanun (LVW) agar, we maintained 100 pathogenic Leptospiraisolates for 12 months without the need for subculture and confirmed the viability of all isolates by the naked eye.

Published
2014
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19. Simultaneous Infection with More than One Strain of Burkholderia pseudomalleiIs Uncommon in Human Melioidosis

Author

Limmathurotsakul, Direk, Wuthiekanun, Vanaporn, Chantratita, Narisara, Wongsuvan, Gumphol, Thanwisai, Aunchalee, Biaklang, Mayurachat, Tumapa, Sarinna, Lee, Sue, Day, Nicholas P. J., and Peacock, Sharon J.

Abstract

ABSTRACTA prospective study was performed to determine the rate at which patients with melioidosis are infected with more than one strain of Burkholderia pseudomallei. Genotyping of 2,058 bacterial colonies isolated from 215 samples taken from 133 patients demonstrated that mixed infection is uncommon (2/133 cases [1.5%; 95% confidence interval, 0.2 to 5.3%]).

Published
2007
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20. Optimization of Culture of Leptospirafrom Humans with Leptospirosis

Author

Wuthiekanun, Vanaporn, Chierakul, Wirongrong, Limmathurotsakul, Direk, Smythe, Lee D., Symonds, Meegan L., Dohnt, Michael F., Slack, Andrew T., Limpaiboon, Roongreung, Suputtamongkol, Yupin, White, Nicholas J., Day, Nicholas P. J., and Peacock, Sharon J.

Abstract

ABSTRACTA prospective study of 989 patients with acute febrile illness was performed in northeast Thailand to define the yield of Leptospirafrom four different types of blood sample. Based on a comparison of the yields from whole blood, surface plasma, deposit from spun plasma, and clotted blood samples from 80 patients with culture-proven leptospirosis, we suggest a sampling strategy in which culture is performed using whole blood and deposit from spun plasma.

Published
2007
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21. Antibodies from Patients with Melioidosis Recognize Burkholderia malleibut Not Burkholderia thailandensisAntigens in the Indirect Hemagglutination Assay

Author

Tiyawisutsri, Rachaneeporn, Peacock, Sharon J., Langa, Sayan, Limmathurotsakul, Direk, Cheng, Allen C., Chierakul, Wirongrong, Chaowagul, Wipada, Day, Nicholas P. J., and Wuthiekanun, Vanaporn

Abstract

ABSTRACTThe indirect hemagglutination assay routinely used to detect antibodies to Burkholderia pseudomalleiwas modified to detect cross-reactivity of antibodies to B. pseudomallei, B. mallei, and B. thailandensisantigens. We demonstrate a lack of cross-reactivity between B. pseudomalleiand B. thailandensisbut marked cross-reactivity between B. pseudomalleiand B. mallei.

Published
2005
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