1. Proposed quality control guidelines for antimicrobial susceptibility tests using tilmicosin.
- Author
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Shryock TR, White DW, Werner CS, and Staples JM
- Subjects
- Animals, Cattle, Cattle Diseases drug therapy, Mannheimia haemolytica drug effects, Microbial Sensitivity Tests methods, Microbial Sensitivity Tests standards, Pasteurella Infections drug therapy, Pasteurella Infections veterinary, Pasteurella multocida drug effects, Quality Control, Reference Standards, Respiratory Tract Infections drug therapy, Respiratory Tract Infections veterinary, Staphylococcus aureus drug effects, Tylosin standards, Tylosin therapeutic use, Anti-Bacterial Agents, Guidelines as Topic, Macrolides, Microbial Sensitivity Tests veterinary, Tylosin analogs & derivatives
- Abstract
Quality control guidelines for tilmicosin, a novel veterinary-use-only macrolide, were developed in a multi-laboratory study according to established National Committee for Clinical Laboratory Standards (NCCLS) procedures (M23-T2). Tilmicosin was incorporated into Sensititre plates for broth microdilution endpoint testing and into two lots of 15-micrograms disks for Kirby-Bauer agar disk diffusion testing. One common lot and five unique lots of Mueller-Hinton media were used. (Broth was cation adjusted, and agar was supplemented with 5% defibrinated sheep blood.) Bacteria used for reference strains included Pasteurella haemolytica 128K, Pasteurella multocida ATCC 43137, and Staphylococcus aureus ATCC 29213 (microdilution) and ATCC 25923 (disk). Replicate tests were conducted. Disk diffusion and broth microdilution quality control ranges are proposed.
- Published
- 1995
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