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Start Over Author "Wai-Hong Wu" Journal journal of clinical microbiology
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1. Comparison of Real-Time, Quantitative PCR with Molecular Beacons to Nested PCR and Culture Methods for Detection of Mycobacterium avium subsp. paratuberculosis in Bovine Fecal Samples

Author

Jill L. Larsen, Salvatore A. E. Marras, Jane Christopher-Hennings, Eric A. Nelson, Jessica L. Pepper, Wai-Hong Wu, William B. Epperson, and Ying Fang

Subjects
Microbiology (medical), Cattle Diseases, Paratuberculosis, Polymerase Chain Reaction, Sensitivity and Specificity, Clinical Veterinary Microbiology, Microbiology, law.invention, Feces, Molecular beacon, law, medicine, Animals, Polymerase chain reaction, biology, biology.organism_classification, medicine.disease, Culture Media, Mycobacterium avium subsp. paratuberculosis, Real-time polymerase chain reaction, Molecular Probes, Cattle, Molecular probe, Nested polymerase chain reaction, Mycobacterium
Abstract

An automated PCR with fluorescent probes (molecular beacons) detected Mycobacterium avium subsp. paratuberculosis in bovine feces. When the PCR was compared with culture in testing 41 fecal samples, kappa scores of 0.94 to 0.96, a sensitivity of 93 to 96%, and a specificity of 92% were obtained. Results were quantitated by using a standard curve derived from a plasmid containing IS 900 . A minimum quantity of 1.7 × 10 −4 pg of DNA, correlating to 1 to 8 CFU, was detected.

Published
2002
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