1. Effect of granulocyte-colony stimulating factor on spinal cord tissue after experimental contusion injury
- Author
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Ömer Beşalti, Erkan Kaptanoglu, Mustafa F. Sargon, Murat Çalişkan, Zeki Sekerci, Gökhan Serbes, A Metin Sanli, and Kamer Kilinc
- Subjects
medicine.medical_specialty ,Lipid peroxidation ,chemistry.chemical_compound ,Microscopy, Electron, Transmission ,Physiology (medical) ,Internal medicine ,Tissue damage ,medicine ,Animals ,Methylprednisolone Hemisuccinate ,Rats, Wistar ,Spinal cord injury ,Spinal Cord Injuries ,Peroxidase ,biology ,business.industry ,Granulocyte-Macrophage Colony-Stimulating Factor ,General Medicine ,Weight drop ,Spinal cord ,medicine.disease ,Axons ,Mitochondria ,Rats ,Granulocyte colony-stimulating factor ,Neuroprotective Agents ,Endocrinology ,medicine.anatomical_structure ,Neurology ,chemistry ,Myeloperoxidase ,Anesthesia ,biology.protein ,Female ,Surgery ,Lipid Peroxidation ,Neurology (clinical) ,business ,Methylprednisolone sodium succinate - Abstract
The purpose of this study was to investigate the early effects of granulocyte-colony stimulating factor (G-CSF) on myeloperoxidase (MPO) activity, lipid peroxidation (LPO) and ultrastructural findings in rats after spinal cord injury (SCI). We also compared the effects of G-CSF and methylprednisolone sodium succinate (MPSS). Wistar rats were divided into four groups: control, SCI alone (50 g/cm weight drop trauma), SCI+MPSS (30 mg/kg), and SCI+G-CSF (50 μg/kg). Administration of G-CSF and MPSS significantly decreased LPO (p < 0.05) and MPO activity (p < 0.05) in the first 24 hours. MPSS was more effective than G-CSF in reducing LPO (p < 0.05) and in minimizing ultrastructure changes. The results of this study indicate that G-CSF exerts a beneficial effect by decreasing MPO activity and LPO and may reduce tissue damage in the first 24 hours after SCI. Our findings do not exclude the possibility that G-CSF has a protective effect on spinal cord ultrastructure after the first 24 hours following SCI.
- Published
- 2010
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