1. Stromal morphological changes and immunophenotypic features of precancerous lesions and hepatocellular carcinoma.
- Author
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Chen D, Li Z, Zhu W, Cheng Q, Song Q, Qian L, and Zhu JY
- Subjects
- Adult, Aged, Carcinoma, Hepatocellular surgery, Female, Glutamate-Ammonia Ligase analysis, Glypicans analysis, HSP70 Heat-Shock Proteins analysis, Humans, Immunohistochemistry, Immunophenotyping methods, Liver Neoplasms surgery, Male, Middle Aged, Neoplasm Grading, Phenotype, Precancerous Conditions surgery, Predictive Value of Tests, Retrospective Studies, Biomarkers, Tumor analysis, Carcinoma, Hepatocellular chemistry, Carcinoma, Hepatocellular pathology, Liver Neoplasms chemistry, Liver Neoplasms pathology, Precancerous Conditions metabolism, Precancerous Conditions pathology, Stromal Cells chemistry, Stromal Cells pathology
- Abstract
Aims: To evaluate stromal histopathological features and immunostaining expression for differential diagnosis of low- and high-grade dysplastic nodules (HGDN) to early and progressed hepatocellular carcinomas (eHCC, pHCC)., Materials: We evaluated sinusoid capillarisation (SC), solitary artery (SA), ductular reaction (DR), stromal invasion and expression of six biomarkers (GPC3, HSP70, GS, CD34, CK19, EpCAM) in a series of 97 cases., Results: Stromal morphological changes, including SC, DR and SA, exhibited significant differences in differential diagnosis. In one indicator, SC had the best sensitivity (90.00%) and accuracy (85.42%), and SA had the best specificity at 88.89 %. In combinations, SC +and SA +were favourable and optimal. The immunoreactivity of GPC3, HSP70 and GS increased significantly in line with the stepwise progression of hepatocarcinogenesis., Conclusions: Stromal histopathology features are useful for diagnosing HGDN, eHCC and small HCC. The immunostaining panel of GPC3, HSP70 and GS can also be supplementary., Competing Interests: Competing interests: None declared., (© Author(s) (or their employer(s)) 2019. No commercial re-use. See rights and permissions. Published by BMJ.)
- Published
- 2019
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