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Your search keyword '"Graziano, Paolo"' showing total 9 results
Start Over Author "Graziano, Paolo" Journal journal of clinical pathology
9 results on '"Graziano, Paolo"'

1. MMR profile and microsatellite instability status in colorectal mucinous adenocarcinoma with synchronous metastasis: a new clue for the clinical practice.

Author

Parente, Paola, Malapelle, Umberto, Angerilli, Valentina, Balistreri, Mariangela, Lonardi, Sara, Pucciarelli, Salvatore, De Luca, Caterina, Pepe, Francesco, Russo, Gianluca, Vigliar, Elena, Danza, Angela, Scaramuzzi, Fabio, Troncone, Giancarlo, Graziano, Paolo, and Fassan, Matteo

Subjects
DNA mismatch repair, MUCINOUS adenocarcinoma, MICROSATELLITE repeats, METASTASIS
Published
2023
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2. Microsatellite instability evaluation of patients with solid tumour: routine practice insight from a large series of Italian referral centre

Author

Russo, Gianluca, primary, Pepe, Francesco, additional, Pisapia, Pasquale, additional, Palumbo, Lucia, additional, Nacchio, Mariantonia, additional, Vigliar, Elena, additional, Pallante, Pierlorenzo, additional, Parente, Paola, additional, Fassan, Matteo, additional, Graziano, Paolo, additional, Bellevicine, Claudio, additional, Troncone, Giancarlo, additional, Malapelle, Umberto, additional, and Iaccarino, Antonino, additional

Published
2022
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3. MMR profile and microsatellite instability status in colorectal mucinous adenocarcinoma with synchronous metastasis: a new clue for the clinical practice

Author

Parente, Paola, primary, Malapelle, Umberto, additional, Angerilli, Valentina, additional, Balistreri, Mariangela, additional, Lonardi, Sara, additional, Pucciarelli, Salvatore, additional, De Luca, Caterina, additional, Pepe, Francesco, additional, Russo, Gianluca, additional, Vigliar, Elena, additional, Danza, Angela, additional, Scaramuzzi, Fabio, additional, Troncone, Giancarlo, additional, Graziano, Paolo, additional, and Fassan, Matteo, additional

Published
2022
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4. Microsatellite instability evaluation of patients with solid tumour: routine practice insight from a large series of Italian referral centre.

Author

Russo, Gianluca, Pepe, Francesco, Pisapia, Pasquale, Palumbo, Lucia, Nacchio, Mariantonia, Vigliar, Elena, Pallante, Pierlorenzo, Parente, Paola, Fassan, Matteo, Graziano, Paolo, Bellevicine, Claudio, Troncone, Giancarlo, Malapelle, Umberto, and Iaccarino, Antonino

Subjects
DNA mismatch repair, MICROSATELLITE repeats, HEREDITARY nonpolyposis colorectal cancer
Published
2023
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6. Molecular profiling of advanced non-small cell lung cancer in the era of immunotherapy approach: a multicenter Italian observational prospective study of biomarker screening in daily clinical practice

Author

Vavala, Tiziana, primary, Malapelle, Umberto, additional, Veggiani, Claudia, additional, Ludovini, Vienna, additional, Papotti, Mauro, additional, Leone, Alvaro, additional, Graziano, Paolo, additional, Minari, Roberta, additional, Bono, Francesca, additional, Sapino, Anna, additional, Manotti, Laura, additional, Troncone, Giancarlo, additional, Pisapia, Pasquale, additional, Girlando, Salvatore, additional, Buffoni, Lucio, additional, Righi, Luisella, additional, Colantonio, Ida, additional, Bertetto, Oscar, additional, and Novello, Silvia, additional

Published
2021
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8. Molecular profiling of advanced non-small cell lung cancer in the era of immunotherapy approach: a multicenter Italian observational prospective study of biomarker screening in daily clinical practice.

Author

Vavala, Tiziana, Malapelle, Umberto, Veggiani, Claudia, Ludovini, Vienna, Papotti, Mauro, Leone, Alvaro, Graziano, Paolo, Minari, Roberta, Bono, Francesca, Sapino, Anna, Manotti, Laura, Troncone, Giancarlo, Pisapia, Pasquale, Girlando, Salvatore, Buffoni, Lucio, Righi, Luisella, Colantonio, Ida, Bertetto, Oscar, and Novello, Silvia

Subjects
NON-small-cell lung carcinoma, OLDER patients, MEDICAL screening, PROGRAMMED cell death 1 receptors, SMALL cell lung cancer, ANAPLASTIC lymphoma kinase, LONGITUDINAL method
Published
2022
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9. Rapid EGFRevaluation from used H&E, IHC and FISH diagnostic slides with the Idylla platform

Author

Nunnari, Josè, Graziano, Paolo, Muscarella, Lucia Anna, Rossi, Antonio, Grillo, Lucia Rosalba, Montrone, Giuseppe, Di Lorenzo, Angela, Bronzini, Monica, and Leone, Alvaro

Abstract

AimsDiagnostic tumour samples are mandatory for morphologic and molecular diagnosis of non-small cell lung cancer (NSCLC) to establish the best therapeutic approach. In the presence of small tumour tissue sample, the pathologist needs to make responsible choices to achieve a correct diagnosis and save material for subsequent molecular evaluations. Nevertheless, in some instances, the diagnostic process can lead to tissue depletion. The automated Idylla epidermal growth factor receptor (EGFR) mutation test has been developed to rapidly process formalin-fixed paraffin-embedded (FFPE) pathologic material, without previous DNA extraction. This study aimed to test whether this platform is suitable for the reuse of H&E, immunohistochemistry (IHC) and fluorescence in situ hybridisation (FISH) diagnostic slides.MethodsA training set of 19 FFPE tissues with known EGFRstatus was revaluated on H&E slides. Fourteen of them were also tested using IHC and FISH treated specimens. An additional series of 25 H&E, IHC or FISH slides of NSCLC cases tested for EGFRmutation at an external institution was blindly assessed as a validation cohort.ResultsCombining the two sets, 32 of 32 classical ex19dels and p.L858R were correctly identified. Three uncommon mutations (p.G719X, p.L861Q and ex20ins) were also detected. Four discrepancies were related to rare ex19del/ins not included in the Idylla list of detectable mutations. Two p.T790M variants were missed on one FFPE and two H&E slides but were detected using IHC and FISH sections from the same FFPE blocks.ConclusionsThe Idylla EGFRmutation test is highly reliable using differently treated tumour specimens and should be validated in larger studies.

Published
2022
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