1. Comparison of one single-antigen assay and three multi-antigen SARS-CoV-2 IgG assays in Nigeria
- Author
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Nnaemeka C. Iriemenam, Fehintola A. Ige, Stacie M. Greby, Olumide O. Okunoye, Mabel Uwandu, Maureen Aniedobe, Stephnie O. Nwaiwu, Nwando Mba, Mary Okoli, Nwachukwu E. William, Akipu Ehoche, Augustine Mpamugo, Andrew Mitchell, Kristen A. Stafford, Andrew N. Thomas, Temitope Olaleye, Oluwaseun O. Akinmulero, Ndidi P. Agala, Ado G. Abubakar, Ajile Owens, Sarah E. Gwyn, Eric Rogier, Venkatachalam Udhayakumar, Laura C. Steinhardt, Diana L. Martin, McPaul I. Okoye, and Rosemary Audu
- Subjects
SARS-CoV-2 ,Immunoassay ,Pre-pandemic specimens ,Sensitivity ,Specificity ,Nigeria ,Infectious and parasitic diseases ,RC109-216 - Abstract
Objectives: Determining an accurate estimate of SARS-CoV-2 seroprevalence has been challenging in African countries where malaria and other pathogens are endemic. We compared the performance of one single-antigen assay and three multi-antigen SARS-CoV-2 IgG assays in a Nigerian population endemic for malaria. Methods: De-identified plasma specimens from SARS-CoV-2 RT-PCR positive, dried blood spot (DBS) SARS-CoV-2 RT-PCR positive, and pre-pandemic negatives were used to evaluate the performance of the four SARS-CoV-2 assays (Tetracore, SARS2MBA, RightSign, xMAP). Results: Results showed higher sensitivity with the multi-antigen (81% (Tetracore), 96% (SARS2MBA), 85% (xMAP)) versus the single-antigen (RightSign (64%)) SARS-CoV-2 assay. The overall specificities were 98% (Tetracore), 100% (SARS2MBA and RightSign), and 99% (xMAP). When stratified based on
- Published
- 2023
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