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Your search keyword '"J. Fabijan"' showing total 4 results
Start Over Author "J. Fabijan" Journal journal of comparative pathology
4 results on '"J. Fabijan"'

1. Pathological Findings in Koala Retrovirus-positive Koalas (Phascolarctos cinereus) from Northern and Southern Australia

Author

Darren J. Trott, Lucy Woolford, Farhid Hemmatzadeh, Nishat Sarker, Helen Owen, Richard D. Emes, Joanne Meers, Greg Simmons, Natasha Speight, Jennifer M. Seddon, Rachael E. Tarlinton, and J. Fabijan

Subjects
040301 veterinary sciences, 030308 mycology & parasitology, Pathology and Forensic Medicine, 0403 veterinary science, Pathogenesis, 03 medical and health sciences, Phascolarctos cinereus, biology.animal, South Australia, Chlamydia pecorum, medicine, Animals, 0303 health sciences, Chlamydia, General Veterinary, biology, Australia, 04 agricultural and veterinary sciences, biology.organism_classification, medicine.disease, Tumor Virus Infections, Lymphatic system, Real-time polymerase chain reaction, Immunology, Koala retrovirus, Gammaretrovirus, Phascolarctidae, Viral load, Retroviridae Infections
Abstract

Koala retrovirus (KoRV) infection shows differences in prevalence and load between northern and southern Australian koala populations; however, the effect of this on diseases such as lymphoma and chlamydial disease is unclear. This study compared clinicopathological findings, haematology and splenic lymphoid area of KoRV-positive koalas from northern (Queensland [Qld], n = 67) and southern (South Australia [SA], n = 92) populations in order to provide further insight into KoRV pathogenesis. Blood was collected for routine haematology and for measurement of KoRV proviral load by quantitative polymerase chain reaction (qPCR). Plasma samples were assessed for KoRV viral load by reverse transcriptase qPCR and conjunctival and cloacal swabs were collected for measurement of the load of Chlamydia pecorum (qPCR). During necropsy examination, spleen was collected for lymphoid area analysis. Lymphoma was morphologically similar between the populations and occurred in koalas with the highest KoRV proviral and viral loads. Severe ocular chlamydial disease was observed in both populations, but urinary tract disease was more severe in Qld, despite similar C. pecorum loads. No associations between KoRV and chlamydial disease severity or load were observed, except in SA where viral load correlated positively with chlamydial disease severity. In both populations, proviral and viral loads correlated positively with lymphocyte and metarubricyte counts and correlated negatively with erythrocyte and neutrophil counts. Splenic lymphoid area was correlated positively with viral load. This study has shown further evidence for KoRV-induced oncogenesis and highlighted that lymphocytes and splenic lymphoid tissue may be key sites for KoRV replication. However, KoRV infection appears to be highly complex and continued investigation is required to fully understand its pathogenesis.

Published
2019

2. Lymphoma, Koala Retrovirus Infection and Reproductive Chlamydiosis in a Koala (Phascolarctos cinereus)

Author

J. Fabijan, Farhid Hemmatzadeh, Lucy Woolford, Natasha Speight, Darren J. Trott, Greg Simmons, and S. Lathe

Subjects
0301 basic medicine, medicine.medical_specialty, Pathology, Lymphoma, Population, Pathology and Forensic Medicine, 03 medical and health sciences, Phascolarctos cinereus, biology.animal, medicine, Mesenteric lymph nodes, Animals, Phascolarctidae, education, education.field_of_study, Chlamydia, General Veterinary, biology, Australia, Chlamydia Infections, medicine.disease, biology.organism_classification, 030104 developmental biology, medicine.anatomical_structure, Koala retrovirus, Histopathology, Female, Retroviridae Infections
Abstract

Koala retrovirus (KoRV) infection, thought to be associated with lymphoid neoplasia, and Chlamydia pecorum-related ocular and urogenital disease are both highly prevalent in eastern Australian koala (Phascolarctos cinereus) populations. However, in South Australian koalas, little is known about KoRV infection and C. pecorum-associated disease. We report the first South Australian case of lymphoma in a KoRV-A-positive female koala also affected by severe reproductive chlamydiosis. The koala was from the Mount Lofty Ranges population and was presented with hindlimb lameness. Clinical examination identified right stifle crepitus, enlarged superficial lymph nodes and paraovarian cysts. Necropsy examination revealed extensive cartilage degeneration and loss over the medial femoral condyle, solid femoral bone marrow, mesenteric and ovarian tumours, paraovarian cysts and purulent metritis. Histopathology confirmed lymphoma in the bone marrow, mesenteric lymph nodes and ovary, with infiltration and parenchymal effacement in the pancreas, adrenal glands and other tissues. Lymphoma, KoRV and chlamydiosis are being investigated further in this population.

Published
2017

3. Pathological Findings in Koala Retrovirus-positive Koalas (Phascolarctos cinereus) from Northern and Southern Australia.

Author

Fabijan J, Sarker N, Speight N, Owen H, Meers J, Simmons G, Seddon J, Emes RD, Tarlinton R, Hemmatzadeh F, Woolford L, and Trott DJ

Subjects
Animals, Australia, Gammaretrovirus, South Australia, Phascolarctidae virology, Retroviridae Infections veterinary, Tumor Virus Infections veterinary
Abstract

Koala retrovirus (KoRV) infection shows differences in prevalence and load between northern and southern Australian koala populations; however, the effect of this on diseases such as lymphoma and chlamydial disease is unclear. This study compared clinicopathological findings, haematology and splenic lymphoid area of KoRV-positive koalas from northern (Queensland [Qld], n = 67) and southern (South Australia [SA], n = 92) populations in order to provide further insight into KoRV pathogenesis. Blood was collected for routine haematology and for measurement of KoRV proviral load by quantitative polymerase chain reaction (qPCR). Plasma samples were assessed for KoRV viral load by reverse transcriptase qPCR and conjunctival and cloacal swabs were collected for measurement of the load of Chlamydia pecorum (qPCR). During necropsy examination, spleen was collected for lymphoid area analysis. Lymphoma was morphologically similar between the populations and occurred in koalas with the highest KoRV proviral and viral loads. Severe ocular chlamydial disease was observed in both populations, but urinary tract disease was more severe in Qld, despite similar C. pecorum loads. No associations between KoRV and chlamydial disease severity or load were observed, except in SA where viral load correlated positively with chlamydial disease severity. In both populations, proviral and viral loads correlated positively with lymphocyte and metarubricyte counts and correlated negatively with erythrocyte and neutrophil counts. Splenic lymphoid area was correlated positively with viral load. This study has shown further evidence for KoRV-induced oncogenesis and highlighted that lymphocytes and splenic lymphoid tissue may be key sites for KoRV replication. However, KoRV infection appears to be highly complex and continued investigation is required to fully understand its pathogenesis., (Copyright © 2020 Elsevier Ltd. All rights reserved.)

Published
2020
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4. Lymphoma, Koala Retrovirus Infection and Reproductive Chlamydiosis in a Koala (Phascolarctos cinereus).

Author

Fabijan J, Woolford L, Lathe S, Simmons G, Hemmatzadeh F, Trott DJ, and Speight N

Subjects
Animals, Australia, Female, Chlamydia Infections veterinary, Lymphoma veterinary, Phascolarctidae, Retroviridae Infections veterinary
Abstract

Koala retrovirus (KoRV) infection, thought to be associated with lymphoid neoplasia, and Chlamydia pecorum-related ocular and urogenital disease are both highly prevalent in eastern Australian koala (Phascolarctos cinereus) populations. However, in South Australian koalas, little is known about KoRV infection and C. pecorum-associated disease. We report the first South Australian case of lymphoma in a KoRV-A-positive female koala also affected by severe reproductive chlamydiosis. The koala was from the Mount Lofty Ranges population and was presented with hindlimb lameness. Clinical examination identified right stifle crepitus, enlarged superficial lymph nodes and paraovarian cysts. Necropsy examination revealed extensive cartilage degeneration and loss over the medial femoral condyle, solid femoral bone marrow, mesenteric and ovarian tumours, paraovarian cysts and purulent metritis. Histopathology confirmed lymphoma in the bone marrow, mesenteric lymph nodes and ovary, with infiltration and parenchymal effacement in the pancreas, adrenal glands and other tissues. Lymphoma, KoRV and chlamydiosis are being investigated further in this population., (Copyright © 2017 Elsevier Ltd. All rights reserved.)

Published
2017
Full Text
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