1. Anti-inflammatory activity of Buchholzia coriacea Engl. (Capparaceae) leaf extract: evaluation of components of the inflammatory response involved
- Author
-
Collins Azubuike Onyeto, Florence N. Mbaoji, Charles Ogbonnaya Okoli, A. C. Ezike, Blessing E. Attah, Stella O. Amanambu, and I. A. Nwabunike
- Subjects
Leukocyte migration ,Erythrocytes ,Hot Temperature ,medicine.drug_class ,Anti-Inflammatory Agents ,Inflammation ,Vascular permeability ,Pharmacology ,Capparaceae ,Hemolysis ,Anti-inflammatory ,Capillary Permeability ,Rats, Sprague-Dawley ,Leukocyte Count ,Mice ,medicine ,Leukocytes ,Animals ,Acetic Acid ,Sheep ,biology ,Plant Extracts ,Cell Membrane ,Complement System Proteins ,medicine.disease ,biology.organism_classification ,In vitro ,Complement system ,Plant Leaves ,Complementary and alternative medicine ,Biochemistry ,medicine.symptom ,Phytotherapy - Abstract
BACKGROUND Earlier studies in our laboratory demonstrated the anti-inflammatory activity of Buchholzia coriacea Engl. (Capparaceae) leaf extract, a herbal remedy used to treat disorders of inflammation. This study was undertaken to evaluate its anti-inflammatory mechanism(s). METHODS The effects of methanol leaf extract of B. coriacea (200 and 400 mg/kg) on vascular permeability and leukocyte migration were studied in rodents, while activity on complement system and membrane stabilization were evaluated in vitro. RESULTS The extract (200 and 400 mg/kg) inhibited acetic acid-induced increase in vascular permeability in a non-dose-related manner and significantly (p < 0.05) reduced the total and differential leukocyte counts, respectively, in a dose-related manner. It also significantly (p < 0.05) inhibited complement-induced hemolysis of sheep red blood cells (40-72%) and moderately inhibited heat- (6%) and hypotonic solution-(24%) induced hemolysis in vitro in a non-dose-related manner. CONCLUSIONS Results demonstrated that the anti-inflammatory activity of B. coriacea leaf extract is mediated through inhibition of increase in vascular permeability, leukocyte migration and complement system, and enhanced membrane stabilization.
- Published
- 2014