1. Photothermal cancer immunotherapy by erythrocyte membrane-coated black phosphorus formulation.
- Author
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Liang X, Ye X, Wang C, Xing C, Miao Q, Xie Z, Chen X, Zhang X, Zhang H, and Mei L
- Subjects
- Animals, Apoptosis drug effects, CD8-Positive T-Lymphocytes drug effects, CD8-Positive T-Lymphocytes microbiology, Cell Line, Tumor, Combined Modality Therapy, Dendritic Cells drug effects, Dendritic Cells immunology, Female, Humans, Mice, Inbred BALB C, Neoplasms immunology, Phosphorus pharmacokinetics, Erythrocyte Membrane, Immunotherapy, Lasers, Neoplasms therapy, Phosphorus administration & dosage, Phototherapy, Quantum Dots administration & dosage
- Abstract
Basal-like breast cancer exhibits a triple-negative phenotype and has a poor prognosis, even with traditional chemical and anti-human epidermal growth factor receptor (HER) treatments. However, the high mutation rate of this obstinate cancer type renders it suitable for immunotherapy. Photothermal therapy (PTT) is a high-efficiency method for inducing tumor neoantigen release in situ, which has great potential for use in cancer immunotherapy. Here, we prepared a biomimetic black phosphorus quantum dot (BPQDs) formulation to induce breast cancer cell apoptosis in situ by near-infrared (NIR) laser irradiation to mobilize the immune system to eliminate the residual and metastatic cancer cells. Erythrocyte membranes (RMs) were used to coat the BPQDs, forming a BPQD-RM nanovesicle (BPQD-RMNV) biomimetic formulation that exhibited a long circulation time and tumor accumulation in vivo. The basal-like 4T1 breast tumor underwent apoptosis and necrosis with the irradiation and recruited dendritic cells (DCs) to capture the tumor antigens in vivo. Furthermore, programmed cell death protein 1 (PD-1) antibody (aPD-1) was employed to prevent the CD8
+ T cells from exhaustion. Notably, BPQD-RMNV-mediated PTT combined with aPD-1 treatment significantly delayed residual and metastatic tumor growth in vivo. Hence, BPQD-RMNV-mediated PTT combined with immune checkpoint blockade antibody increased the infiltration and activity of CD8+ T cells in the tumor, which directly restrained basal-like breast tumor growth in vivo., (Copyright © 2019. Published by Elsevier B.V.)- Published
- 2019
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